DNA coding for alkaline protease

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor

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43525233, 4353201, 536 241, C12N 1531, C12N 1563, C12N 1574, C12N 1570

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active

055433227

ABSTRACT:
There are disclosed cloning of an alkaliprotease secretion gene of a mold and a DNA fragment containing a part or all of the alkaliprotease gene. A plasmid into which the DNA fragment is integrated, a transformant obtained by using the plasmid and the production of a useful protein by using the transformant are also disclosed.

REFERENCES:
patent: 3655570 (1972-04-01), Isono et al.
Journal of The Takeda Research Laboratories vol. 38, No. 1-2, 1979, JP pp. 33-43, K. Tomodo et al. "Production, purification and general properties of Fusarium alkaline protease."
Biochemical and Biophysical Research Communications vol. 145, No. 2, Jun. 15, 1987, New York pp. 712-718, K. Yoshimura et al. "Differences between Saccharomyces cerevisiae and Bacillus subtilis in secrection of human lysozyme."
Database WPIL Week 8841, Derwent Publications Ltd., London, GB; An 88-290577 (41) & JP-A-63 214 187 (Mitsubishi Gas Chem KK) Sep. 6, 1988 *abstract*.
Journal of the Takeda Research Laboratories vol. 38 "Production, Purification and General Properties of Fusarium Alkaline Protease" (1979) Tomoda et al. pp. 33-43.
Agriculture and Biological Chemistry vol. 38 "Screening of Fungi Producing Alkaline Protease From n-Paraffins and Iduction of Kabicidin Resistant Mutant of Fusarium sp" (1974) Suzuki et al. pp. 135-139.
Biotechnology, Dec. 1988, vol. 6 No. 12, "High Level Expression of Recombinant Genes in Aspergillus Oryzae" Christensen et al. pp. 1419-1422.
Database WPIL Week 9040, Derwent Publications Ltd., London, GB; An 90-301017 (40) & JP-A-2 211 869 Shokuhin Sangyo Kos) 23 Aug. 1990 *abstract*.
Tomoda et al (1979) J. Takeda Res Lab 38:33-43.
Tatsumi (1989) Mol Gen Genet 219:33-38.
Igogai (Feb. 1991) Agric Biol Chem 55:471-477.
Lee (1988) Science 239:1288-1291.

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