Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1996-06-14
2000-02-15
Eisenschenk, Chris
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 691, 4352523, 4353201, 530350, 530403, 536 2372, 4241991, 4242041, C12P 2106, C12N 1500
Patent
active
060251638
DESCRIPTION:
BRIEF SUMMARY
I. FIELD OF THE INVENTION
The present invention relates to a DNA encoding a peptide of a papilloma virus major capsid protein. Furthermore, this invention concerns a papilloma virus genome containing such a DNA. In addition, this invention relates to proteins encoded by the papilloma virus genome and to virus-like particles as well as antibodies directed thereagainst and the use thereof in diagnosis, treatment and vaccination.
II. BACKGROUND OF THE INVENTION
It is well known that papilloma viruses infect the epithelial tissue of humans and animals. Human papilloma viruses (referred to as HP viruses below) are found in benign, e.g., warts, condylomata in the genital region, and malign, e.g., carcinomas of the skin and uterus, epithelial neoplasms. Zur Hausen, 1989, Cancer Research 49:4677-4681. HP viruses are also considered for the development of malign tumors of the respiratory tract. Zur Hausen, 1976, Cancer Research 36:530. In addition, HP viruses are considered at least co-responsible for the development of squamous carcinomas of the lungs. Syrjanen, 1980, Lung 158:131-142.
Papilloma viruses have an icosahedral capsid without coat, which includes a circular, double-stranded DNA molecule of about 7,900 bp. The capsid comprises a major capsid protein (L1) and a minor capsid protein (L2). Both proteins, coexpressed or L1 expressed alone, result in vitro to the development of virus-like particles. Kirnbauer et al., 1993, Journal of Virology 67:6929-6936.
Papilloma viruses cannot be proliferated in monolayer cell culture. Therefore, their characterization is extremely difficult, the detection of papilloma viruses already creating considerable problems. This applies particularly to papilloma viruses in carcinomas of the skin. A reliable detection thereof and thus well-calculated steps thereagainst have not been possible by now.
Therefore, it is the object of the present invention to provide an agent serving for detecting papilloma viruses, particularly in carcinomas of the skin. Furthermore, an agent is to be provided which serves for taking therapeutic steps against these papilloma viruses.
According to the inventions this is achieved by the provision of the subject matters in the claims.
III. SUMMARY OF THE INVENTION
The present invention is directed to a DNA encoding a peptide of a papilloma virus major capsid protein.
The present invention is also directed to a papilloma virus genome containing such a DNA.
The present invention is further directed to proteins encoded by the papilloma virus genome and to virus-like articles as well as antibodies directed thereagainst and the use thereof in diagnosis, treatment and vaccination.
IV. BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1 shows the base sequence and the amino acid sequence, derived therefrom, of a DNA encoding a peptide of L1 of a papilloma virus (SEQ ID NO:1). This DNA was deposited as plasmid VS93-1 with the DSM (German Collection of Microorganisms and Cell Cultures) under DSM 9133 on Apr. 12, 1994.
FIG. 2 shows the base sequence and the amino acid sequence, derived therefrom, of an DNA encoding a peptide of L1 of a papilloma virus (SEQ ID NO:2). This DNA was deposited as plasmid CR148-59 with the DSM under DSM 9134 on Apr. 12, 1994.
FIG. 3 shows the base sequence and the amino acid sequence, derived therefrom, of an DNA encoding a peptide of L1 of a papilloma virus (SEQ ID NO:3). This DNA was deposited as plasmid VS40-7 with the DSM under DSM 9135 on Apr. 12, 1994.
FIG. 4 shows the base sequence and the amino acid sequence, derived therefrom, of an DNA encoding a peptide of L1 of a papilloma virus (SEQ ID NO:4). This DNA was deposited as plasmid VS20-4 with the DSM under DSM 9136 on Apr. 12, 1994.
FIG. 5 shows the base sequence and the amino acid sequence, derived therefrom, of an DNA encoding a peptide of L1 of a papilloma virus (SEQ ID NO:5). This DNA was deposited as plasmid VS102-4 with the DSM under DSM 9137 on Apr. 12, 1994.
FIG. 6 shows the base sequence and the amino acid sequence, derived therefrom, of an DNA encoding a peptide of L1
REFERENCES:
Shamanin, et al., Sep. 1994, "Specific types of human papillomavirus found n benign proliferations and carcinomas of the skin in immunosuppressed patients, " Cancer Research 17:4610-4613 .
Zur Hausen, 1989, Cancer Research 49:4677-4681.
Zur Hausen, 1976, Cancer Research 36:530.
Syrjanen, 1980, Lung 158;131-142.
Kirnbauer, et al., 1993, J. Virology6929-6936.
Hagensee, et al., 1993, J. Virology315-322.
Rose et al. 1993, J. of Virology, vol. 76, No. 4, pp. 1936-1944, Apr. 1993.
Tomita et al. 1987, J. of Virology, vol. 61, No. 8, pp. 2389-2394, Aug.1987.
Tomita et al. 1987, Virology, vol. 158, pp. 8-14, 1987.
Volpers et al. 1991, Virology, vol. 181, pp. 419-423, 1991.
De Villiers-Zur Hausen Ethel-Michele
Hausen Harald Zur
Leigh Irene
Shamanin Vladimir
Deutsches Krebsforschungzentrum Stiftung des Offentlichen Rechts
Eisenschenk Chris
Salimi Ali R.
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