DNA cloning vectors with in vivo excisable plasmids

Details DNA cloning vectors with in vivo excisable plasmids DNA cloning vectors with in vivo excisable plasmids

1992-05-21

1994-02-15

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

4353201, C12N 1510, C12N 1570

Patent

active

052866360

ABSTRACT:
Vectors are described that circumvent traditional DNA cloning and subcloning procedures, and that contain a unique DNA cartridge that permits both cloning of DNA directly into DNA sequences present within the cartridge, and in vivo removal and circularization of the cartridge thereby yielding an autonomously replicating structure. Because the DNA cartridge can include a wide variety of functional DNA sequences, the cloned DNA can be subjected to a plethora of molecular biological procedures without having to remove the cloned DNA from the cartridge thereby obviating the need to perform additional subcloning techniques. A particularly useful example of this type of vector is bacteriophage lambda containing the DNA cartridge.

REFERENCES:
patent: 5128256 (1992-07-01), Huse et al.

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