Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2007-03-13
2007-03-13
Kam, Chih-Min (Department: 1656)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S007210, C435S007230, C435S069100, C435S235100, C436S501000, C536S023100, C536S023400, C536S023500, C536S023600, C536S023700
Reexamination Certificate
active
09518297
ABSTRACT:
The present invention provides molecular switch system methods and compositions for use in regulatable gene expression. The system includes a nucleic acid construct which has a DNA response sequence for a transcriptional regulatory protein operably linked to a promoter, a compound binding sequence in the vicinity of the DNA response sequence, a transgene under the control of the promoter; and a DNA binding compound. In some cases, the molecular switch system further includes a nucleic acid sequence encoding a transcriptional regulatory protein operably linked to a second promoter. The invention further provides a method for screening compounds for the ability to function in the molecular switch system and thereby regulate gene expression.
REFERENCES:
patent: 5071773 (1991-12-01), Evans et al.
patent: 5306619 (1994-04-01), Edwards et al.
patent: 5527690 (1996-06-01), Goldstein et al.
patent: 5578444 (1996-11-01), Edwards et al.
patent: 5693463 (1997-12-01), Edwards et al.
patent: 5716780 (1998-02-01), Edwards et al.
patent: 5726014 (1998-03-01), Edwards et al.
patent: 5738990 (1998-04-01), Edwards et al.
patent: 5744131 (1998-04-01), Edwards et al.
patent: 5843653 (1998-12-01), Gold et al.
patent: 5869241 (1999-02-01), Edwards et al.
patent: 5869250 (1999-02-01), Cheng et al.
patent: 5874209 (1999-02-01), Karin et al.
patent: 5912411 (1999-06-01), Bujard et al.
patent: WO 93/23431 (1993-11-01), None
patent: WO 94/29442 (1994-12-01), None
patent: WO 96/37609 (1996-11-01), None
patent: WO 96/41865 (1996-12-01), None
patent: WO 97/10337 (1997-03-01), None
patent: WO 98/03646 (1998-01-01), None
patent: WO 99/25856 (1999-05-01), None
patent: WO 00/09704 (2000-02-01), None
Gottesfeld et al., Regulation of Gene Expression by Small Molecules. Nature 387, 202-205 (1997).
Dickinson et al., Inhibition of RNA Polymerase II Transcription in Human Cells by Synthetic DNA-Binding Ligands. Proc. natl. Acad. Sci USA 95 12890-12895 (1998).
Voet etal., Biochemistry pp. 854-856 and 868 (1990).
Brar, D.S., et al., “Genetically Engineered Plants for Quality Improvement”Biotechnology and Genetic Engineering Reviews 13:167-179 (1995).
Delort, J.P., and Capecchi, M.R., “TAXI/UAS: A Molecular Switch to Control Expression of Genes In Vivo”Human Gene Therapy 7:809-820 (1996).
Gossen, M., et al., “Transcriptional Activation by Tetracycines in Mammalian Cells”Science 268:1766-1769 (1995).
Martin, G.B., “Gene discovery for crop improvement”Current Opinion in Biotechnology 9:220-226 (1998).
Miller, N., and Whelan, J., “Progress in Transcriptionally Targeted and Regulatable Vectors for Genetic Therapy”Human Gene Therapy 8:803-815 (1997).
Moore, I., et al., “A transgenic activation system for regulated gene expression in transgenic plants”Proc. Natl. Acad. Sci. USA 95:376-381 (1998).
Pomerantz, J.L., et al., “Structure-Based Design of Transcription Factors”Science 267:93-96 (1995).
Walther, W., and Stein, U., “Cell type specific and inducible promoters for vectors in gene therapy as an approach for cell targeting”J. Mol Med 74:379-392 (1996).
Bruice Thomas W.
Edwards Cynthia A.
Fry Kirk E.
Kwok Yan
Laurance Megan E.
Genelabs Technologies, Inc.
Kam Chih-Min
Perkins Coie LLP
LandOfFree
DNA binding compound-mediated molecular switch system does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with DNA binding compound-mediated molecular switch system, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and DNA binding compound-mediated molecular switch system will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3766333