Chemistry: electrical and wave energy – Apparatus – Electrophoretic or electro-osmotic apparatus
Patent
1995-07-10
1996-09-17
Niebling, John
Chemistry: electrical and wave energy
Apparatus
Electrophoretic or electro-osmotic apparatus
204461, 204466, 204467, 204616, 204618, 356344, G01N 2726, G01N 27447
Patent
active
055565298
ABSTRACT:
The improved DNA base sequencer has a flat plate type gel electrophoretic unit that has multiple tracks for electrophoresing DNA fragments and which is held in a vertical position, a light exciting laser light applying unit that applies laser light to the respective tracks in the electrophoretic unit from one lateral side thereof in such a way that it crosses the tracks at right angles, and a fluorescence detecting unit that detects the fluorescence as generated from the DNA fragments illuminated with the laser light and which converts the detected fluorescence to an electric signal. The sequencer is characterized in that the fluorescence detecting unit comprises a fluorescence condensing lens, a fluorescence filtering unit and a solid-state imaging device, (e.g., a CCD line sensor), the fluorescence filtering unit being composed of at least two filters that selectively transmit fluorescences having different wavelengths and that are staggered with each other along a common longitudinal axis. The sequencer achieves a sensitivity and resolution at least comparable to those attained by the prior art apparatus and yet which is capable of detecting multi-color labelled samples by means of simpler detection optics.
REFERENCES:
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patent: 5290419 (1994-03-01), Kambara et al.
patent: 5307148 (1994-04-01), Kambara et al.
Bruno et al, "The Pigtailing Approach to Optical Detection in Capillary Electrophoresis", TrAC: Trends in Analytical Chemistry, vol. 13, No. 5, May 1994, pp. 190-198.
Hitachi Electronics Engineering Co. Ltd.
Niebling John
Starsiak Jr. John S.
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