Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Lyase
Reexamination Certificate
2005-10-04
2005-10-04
Rao, Manjunath N. (Department: 1652)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Lyase
C435S004000, C435S006120, C435S069100, C435S136000, C435S146000, C435S183000, C435S232000, C435S252300, C435S320100, C435S410000, C536S023200
Reexamination Certificate
active
06951751
ABSTRACT:
A novel tyrosine-inducible tyrosine ammonia lyase enzyme was isolated from the yeastTrichosporon cutaneum. This enzyme has a higher activity for tyrosine than for phenylalanine and is useful for the production of para-hydroxycinnamic acid directly from tyrosine. The gene encoding this enzyme was sequenced using 3′ and 5′ RACE cloning of the TAL cDNA and the gene was expressed in the yeastSaccharomyces cerevisiaeand in the bacteriumEscherichia coli.
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Breinig Sabine
Qi Wei Wei
Sariaslani Fateme Sima
Vannelli Todd M.
Xue Zhixiong
E. I. du Pont de Nemours and Company
Fronda Christian L.
Rao Manjunath N.
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