DNA analyzing method

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 5, 435 911, 435 912, 536 243, 536 2432, 536 2433, 204456, 204461, C12Q 168, C12Q 170, C12P 1934, C07H 2104

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056502745

ABSTRACT:
A DNA analyzing method comprising ligating the oligomer of a known base sequence to the DNA fragment obtained by digesting a sample with a restriction enzyme, hybridizing an oligomer and DNA fragment using oligomers which have the sequences of all combinations of the types of the bases within the length of several bases following the known base sequence, checking the presence or absence of hybridization or complementary DNA strand extension, identifying the DNA fragment terminal sequence from this result, and fractionating the DNA fragments and analyzing them. This DNA analyzing method provides an effective analysis of mixtures of long DNAs or DNA fragments.

REFERENCES:
patent: 5188963 (1993-02-01), Stapleton
A.B. Chetverin et al., Oligonucleotide Arrays: New Concept and Possibilities, Biotechnology, vol. 12, Nov. 1994, pp. 1093-1099.
S. Brenner et al., DNA Fingerprinting by Sampled Sequencing, Proceedings of the National Academy of Sciences of USA, vol. 86, 1989, pp. 8902-8906.
S.P.A. Fodor et al., Light-Directed, Spatially Addressable Parallel Chemical Synthesis, Science, vol. 251, No. 4995, Feb. 15, 1991, pp. 767-773.
Southern et al. Genomics 13: 1008-1017 1994.
Southern et al. J. of Biotechnology 35:217-227 1994.
Khrapko et al. FEBS 256: 118-122 1989.
Chetverin et al. Biosystems 30: 215-31 1993.
Pevzner et al. J. of Biomolecular Structure and Dynamics 9 399-409 1991.

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