DNA amplification with thermostable DNA polymerase and polymeras

Details DNA amplification with thermostable DNA polymerase and polymeras DNA amplification with thermostable DNA polymerase and polymeras

1992-10-07

1994-08-16

Parr, Margaret

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, 53038826, 5303891, C12P 1934, C12Q 168, C07K 1528

Patent

active

053386710

ABSTRACT:
Antibodies which are specific to a thermostable DNA polymerase can be used to reduce or eliminate the formation of non-specific products in polymerase chain reaction methods. These antibodies and other temperature sensitive inhibitors are effective to inhibit DNA polymerase enzymatic activity at a certain temperature T.sub.1 which is generally below about 85.degree. C. The inhibitors are irreversibly inactivated at temperature T.sub.2 which is generally above about 40.degree. C. T.sub.2 is also greater than T.sub.1. Such inhibitors can be supplied individually or in admixture with the DNA polymerase in a diagnostic test kit suitable for PCR.

REFERENCES:
patent: 4638028 (1987-01-01), Lui et al.
patent: 4889818 (1989-12-01), Gelfand et al.
patent: 4965188 (1990-10-01), Mullis et al.
patent: 5210036 (1993-05-01), Comb et al.
Bobrovnik, S. A. et al. Dynamics and mechanism of antibody thermoinactivation. Biopolim. Kletka. (1988) 4:310-313 (Abstract).

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