DNA amplification technique

Details DNA amplification technique DNA amplification technique

1989-01-03

1991-02-19

Wax, Robert A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 91, 435 18, 435199, 435 15, 435193, 436501, 436 94, C12Q 168, C12P 1934, C12N 922, G01N 33566

Patent

active

049943707

ABSTRACT:
A modification of the polymerase chain reaction (PCR) technique is described. The method allows the amplification of regions of DNA flanking a single region of known sequence, in contrast to standard PCR which requires two regions of known sequence at opposite ends of the fragment to be amplified. Various advantages of the new method are described.

REFERENCES:
patent: 4693979 (1987-09-01), Stetter et al.
patent: 4800159 (1989-01-01), Mullis et al.
Maniatis et al., Molecular Cloning, a Lab. Manual, Cold Spring Harbor Lab., 1982.
Davis, L. G et al., Basic Methods in Molecular Biology, Elsevier, N.Y., 1986. (contents pp.).
Davis, R. W. et al., A Manual for Genetic Engineering: Advanced Bact. Genetics, Cold Spring Harbor Lab., 1980 (contents pp.).
Ochman, H., et al., Genetics 120: 621-623 (Nov., 1988).
Triglia, T., et al., Nucleic Acids Research, 16, No. 16: 8186 (Aug. 1988).
Silver, J., et al., Cold Spring Harbor Laboratory Meeting on RNA Tumor Viruses, p. 9, May 1988.
Mullis, K. B., "The Polymerase Chain Reaction: Why It Works" in Current Communications in Molecular Biology (Cold Spring Harbor Laboratory Press, H. A. Erlich, Ed.), pp. 237-243, (1989).

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