Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Reexamination Certificate
2002-11-13
2010-02-02
Benzion, Gary (Department: 1637)
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
C435S091100
Reexamination Certificate
active
07655791
ABSTRACT:
The present invention is directed to methods to prepare a DNA molecule or a plurality of DNA molecules by random fragmentation. In some embodiments, the present invention regards preparing a template for DNA sequencing by random fragmentation. In specific embodiments, the random fragmentation comprises chemical fragmentation, mechanical fragmentation, or enzymatic fragmentation. In further specific embodiments, a universal sequence is attached to the 3′ end of the DNA fragments, such as by ligation of an adaptor sequence or by homopolymeric tailing with terminal deoxynucleotidyltransferase. In other embodiments, a library is prepared with methods of the present invention.
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Bruening Eric
Kamberov Emmanuel
Makarov Vladimir L.
Sleptsova Irina
Benzion Gary
Fulbright & Jaworski L.L.P.
Rubicon Genomics, Inc.
Thomas David C
LandOfFree
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