Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Patent
1985-01-02
1987-11-03
Davie, James W.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
435 91, 4351723, 435253, 435320, 935 29, 935 41, 935 60, 935 73, C12P 2100, C12P 1934, C12N 1500, C12N 100
Patent
active
047047208
ABSTRACT:
An expression vector carrying a gene coding for a phosphate-binding protein has been found to have a strong gene expression. The expression vector can be produced by transforming a bacterium belonging to Enterobacteriaceae with a recombinant vector carrying a DNA fragment containing a gene coding for a phosphate-binding protein to form transformants, selecting the transformants containing the desired recombinant vector from said transformants, and isolating the desired recombinant vector from the selected transformants. The expression vector is useful for producing polypeptides.
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Amemura et al, 1981, (Abstract), "Cloning of Alkaline Phosphatase Regulat Genes of E. coli", 4th Ann. Meeting of Japan Mol. Biol.
Maniatis et al, 1982, Molecular Cloning A Laboratory Manual, Cold Spring Harbor Lab., p. 422.
Iwakura et al, 1982, "Isolation of DNA Fragment Containing the Phos Gene of Escherichia coli, K-12", J. Biochem, v92, 615-622.
Amemura et al, 1982, "Cloning of and Complementation Tests with Alkaline Phosphatase Regulatory Genes (phoS and phoT) of E. coli", J. Bact., v152, 692-701.
Levitz, et al., "Complementation Tests Between Alkaline Phosphatase-Constitutive Mutants (phoS and phoT) of E. coli", J. Bact., 1981, vol. 145, pp. 1432-1435.
Zuckier et al., "Genetic and Physiological Tests of Three Phosphate-Specific Transport Mutants of E. coli", J. Bact., 1981, vol. 145, pp. 1249-1256.
Davie James W.
NEC Corporation
The Research Foundation for Microbial Diseases of Osaka Universi
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