Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1994-06-14
1995-12-12
Jones, W. Gary
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
536 241, 536 242, 536 243, 536 2432, 530324, C07H 1700, A61K 500
Patent
active
054750989
ABSTRACT:
The present invention provides isolated nucleic acid sequences corresponding to the hlyA gene, the hlyB gene and the intergenic region between the hlyA gene and the hlyB gene which are present in enterohemorrhagic E. coli. In addition, the present invention provides methods for detecting enterohemorrhagic E. coli by targeting the hlyA gene, the hlyB gene, the intergenic region between the hlyA and the hlyB genes, combinations thereof, or fragments thereof. Such methods rely on nucleic acid probes and amplification primers specific for subsequences of the hlyA gene, the hlyB gene, the intergenic region between the hlyA and the hlyB genes, combination thereof or, fragments thereof. As such, the present provides nucleic acid probes and amplification primers which can be used for the rapid, sensitive and specific amplification and detection of enterohemorrhagic E. coli. In addition, the present invention provides kits embracing the above aspects.
REFERENCES:
patent: 4837306 (1989-06-01), Ling et al.
Hall Robert H.
Xu Jian-Guo
Jones W. Gary
Sisson Bradley L.
The United States of America as represented by the Department of
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