Disruption of the cytochrome C gene in xylose-fermenting yeast

Details Disruption of the cytochrome C gene in xylose-fermenting yeast Disruption of the cytochrome C gene in xylose-fermenting yeast

1999-03-23

2000-06-06

Yucel, Remy

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing oxygen-containing organic compound

435 41, 435 93, 435155, 435161, 4352542, 43525423, C12P 708, C12N 114

Patent

active

060717297

ABSTRACT:
Disclosed is a xylose-fermenting mutant yeast strain exhibiting reduced expression of cytochrome c and enhanced fermentation of xylose relative to xylose-fermenting yeast strains in which cytochrome c is fully functional. Also disclosed is a method of producing ethanol from xylose by culturing a xylose-fermenting mutant yeast strain exhibiting reduced expression of cytochrome c in the presence of xylose-containing material.

REFERENCES:
patent: 5126266 (1992-06-01), Jeffries et al.
Jeppsson et al., "Existence of Cyanide-Insensitive Respiration in the Yeast Pichia stipitis and Its Possible Influence on Product Formation during Xylose Utilization," Applied and Environmental Microbiology, 61:2596-2600 (1995).
Lu et al, "Cloning and disruption of the .beta.-isopropylmalate dehydrogenase gene (LEU2) of Pichia stipitis with URA3 and recovery of the double auxotroph," Appl. Microbiol Biotechnol. 49:141-146 (1998).
Sreenath et al., "Diminished Respirative Growth and Enhanced Assimilative Sugar Uptake Result in Higher Specific Fermentation Rates by the Mutant Pichia stipitis FPL-061," Applied Biochemistry and Biotechnology 63:109-116 (1997).

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