Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1985-12-17
1988-06-21
Warden, Robert J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 1, 436501, 536 26, 536 27, 536 28, 935 77, 935 78, C12Q 168, G01N 33566
Patent
active
047525665
ABSTRACT:
A method for determining the presence and amount of a predetermined target nucleotide sequence in a biological sample is provided. The method employs a reagent complex which consists of a labeled probe polynucleotide having a target binding region and a second polynucleotide bound to the labeled probe in a second polynucleotide binding region thereof. The second binding region is at least partially coextensive with the target binding region. This reagent complex is contacted with a sample under conditions in which the target sequence binds to the labeled probe, displacing the second polynucleotide. Labeled probe/target hybrids are separated from intact reagent complexes and the presence and amount of these hybrids are determined.
REFERENCES:
patent: 4205952 (1980-06-01), Cais
patent: 4563417 (1986-01-01), Albarella et al.
Green, C., et al, Nucleic Acids Research, 9, No. 8: 1905-1918, 1981.
Collins Mary
Dougherty Joseph P.
Bak Mary E.
Eisen Bruce M.
Genetics Institute Inc.
O'Shaughnessy Brian P.
Spiegel Jack
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