Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1984-05-07
1988-08-23
Warren, Charles F.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435803, 435810, 935 78, 436501, C12Q 168, G01N 33566
Patent
active
047660627
ABSTRACT:
A diagnostic reagent is disclosed containing a complex of a probe polynucleotide (P) bound via purine/pyrimidine hydrogen bonding to a labeled polynucleotide (L). The probe (P) contains a target binding region (TBR) capable of binding to a target nucleotide sequence (G) of a biological sample. A method is disclosed in which contact with a sample containing the target nucleotide sequence (G) causes binding, initially between G and a single-stranded portion (IBR) of the target binding region (TBR). Thereafter the labeled polynucleotide (L) is displaced from the complex by branch migration of (G) into the (P)/(L) binding region. Determination of displaced labeled polynucleotide (L) gives a value which is a function of the presence and concentration of target nucleotide sequence (G) in the sample.
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Brewen Joseph G.
Collins Mary
Diamond Steven E.
Ellwood Marian S.
Fritsch Edward F.
Allied Corporation
Doernberg Alan M.
Genetics Institute Inc.
Jay Jeremy M.
Warren Charles F.
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