Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Patent
1995-10-19
1997-02-25
Fleisher, Mindy
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
530412, 530415, 530344, 564292, 564295, C07K 114, C07K 116, C07K 118
Patent
active
056060335
ABSTRACT:
A method for the purification of proteins by displacement chromatography on ion exchange media using low molecular weight displacers is disclosed. Several classes of low molecular weight anionic species are exemplified, including aromatic rings having sulfonic acid or carboxylic acid moieties attached thereon, sulfated sugar derivatives, anionic antibiotics, and dendrimeric polymers. Novel compounds useful as displacers are dendrimers of formula ##STR1## wherein R.sup.1 is lower alkyl, n is 2 to 6 and similar dendritic polymers based thereon.
REFERENCES:
patent: 2946757 (1960-07-01), Butler et al.
patent: 3651231 (1972-03-01), Eppstein et al.
patent: 5028696 (1991-07-01), Torres et al.
patent: 5043423 (1991-08-01), Viscomi et al.
patent: 5156835 (1992-10-01), Nabi et al.
patent: 5478924 (1995-12-01), Cramer et al.
Chemical Abstracts vol. 105: 154826 1986.
Newlcome et al. "Cascade Molecules: A New Approach to Mlcelles" J Org Chem. 50 2003-2004 1985.
Eckstein et al. "Synthesis Vernetzter Lysin -und Lysin Peptidgele" Makromol Chem. 181 2471-2480 1980 Abstract.
"Chromatography" Ed. Heftman pp. A30-A33 1983.
Cramer and Brooks "Ion-Exchange Displacement Chromatography of Proteins" ACS Symposium Series, 27-52 (1993).
Frenz et al. "Investigation of Operating Parameters in High-Performance Displacement Chromatography" J. Chrom., 330, 1-17 (1985).
Valko et al. "Displacement Chromatography of Oligomycins" J. Chrom., 386, 345-351 (1987).
Viscomi et al. "High-Performance Displacement Chromatography of Melanotropins and Their Derivatives" J. Chrom., 440, 157-164 (1988).
Viscomi et al. "Preparative Purification of Plasmodium Falciparum Circumsporozoite Protein . . . " J. Chrom., 482, 99-106 (1989).
Vigh et al. "Displacement Chromatography On Cyclodextrin-Silicas" J. Chrom., 484, 237-250 (1989).
Cardinali et al. "Scaling-Up Procedure From the Range of Milligrams to Grams For The Purification of Amino Acid . . . " J. Chrom., 499 37-45 (1990).
Torres and Peterson "Purification of complex protein mixtures by ion-exchange . . . " J. Chrom., 604, 39-46 (1992).
Kalghatgi et al. "Rapid displacement chromatography of melittin on micropellicular . . . " J. Chrom., 604, 47-53 (1992).
Frenz et al. "Characterization of a Tryptic Digest By High-Performance . . . " J. Chrom., 557, 289-305 (1991).
Viscomi et al. "Large-scale Purification of the Synthetic Peptide Fragment . . . " J. Chrom., 549, 175-184 (1991).
Ghose and Mattiasson "Evaluation of displacement chromatography for the recovery of lactate . . . " J. Chrom., 547, 145-153 (1991).
Verbalis "Heterogeneity of human pituitary neurophysins by ampholyte . . . " J. Chrom., 254, 309-314 (1983).
Liao et al. "High Performance Displacement Chromatography of Proteins: Separation of . . . " Chromatographia, 24, 881-885 (1987).
Jen and Pinto "Use of the sodium salt of poly(vinylsulfonic acid) as a low-molecular-weight displacer . . . " J. Chromatogr., 519, 87-98 (1990).
Jen and Pinto "Dextran Sulfate as a Displacer for the Displacement Chromatography of . . . " J. Chromatogr., 29, 478-484 (1991).
Gerstner and Cramer "Cation-Exchange Displacement Chromatography of Proteins with . . . " Biotechnol. Prog., 8, 540-545 (1992).
Jen and Pinto "Influence of displacer properties on displacement chromatography of . . . " Reactive Polymers, 19, 145-161 (1993).
Jayaraman et al. "Ion-exchange displacement chromatography of proteins . . . " J. Chromtogr., 630, 53-68 (1993).
Cramer Steven M.
Jayaraman Guhan
Kundu Amitava
Li Yufei
Moore James A.
Degen Nancy J.
Fleisher Mindy
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