Dispersion devices for aggregates

Solid material comminution or disintegration – Apparatus – Including means applying fluid to material

Reexamination Certificate

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Details

C241S047000

Reexamination Certificate

active

07658341

ABSTRACT:
The present invention encompasses a flow-through dispersion device for the dispersion of cell aggregates, the device comprising a first or upstream inlet obstacle and a second or downstream outlet obstacle, each obstacle having at least one traversing hole wherein no two holes align. Between the two obstacles is a turbulence chamber, such that when a cell suspension is passed through the inlet holes, into the chamber and then exits the second obstacle, the turbulence within the chamber disrupts and disperses cell aggregates. The device optionally may have more than two obstacles and turbulence chambers and multiple units may be placed in series to increase the amount of turbulence and time applied to a volume of cells. The invention further encompasses methods of using the device to disperse cell aggregates, and methods of culturing cells that involve seeding cultures and maintaining dispersed individual cells.

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patent: 4919339 (1990-04-01), Niemi
patent: 5143303 (1992-09-01), Niemi
patent: 5598979 (1997-02-01), Rowley, Jr.
patent: 6138931 (2000-10-01), Geurts et al.
patent: 6739531 (2004-05-01), Taylor
patent: 6786437 (2004-09-01), Ribardi
patent: 6871806 (2005-03-01), Tsai et al.

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