Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Reexamination Certificate
2011-08-02
2011-08-02
Swope, Sheridan (Department: 1652)
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
C435S320100, C435S252300
Reexamination Certificate
active
07989604
ABSTRACT:
Isolated nucleic acid sequences and amino acid sequences for soluble, β-N-acetylglucosaminidase or active fragments or variants thereof which promote detachment of bacterial cells from a biofilm are provided. An isolated mutant bacteria which forms biofilm colonies which tightly adhere to surface but which are unable to release cells into the medium or spread over the surface is also provided. In additions, methods are described for modulating detachment of bacterial cells from biofilm by mutating soluble, β-N-acetylglucosaminidase or altering its expression or activity are also provided. Also provided are compositions, methods and devices for preventing, inhibiting and treating bacterial infections.
REFERENCES:
patent: 5238843 (1993-08-01), Carpenter et al.
patent: 7064105 (2006-06-01), Joullie et al.
patent: 3-231999 (1991-10-01), None
patent: WO 98/50512 (1998-11-01), None
Galye et al, Identification of regions in interleukin-1 alpha important for activity. J Biol Chem. Oct. 15, 1993;268(29):22105-11.
Whisstock et al, Prediction of protein function from protein sequence and structure. Q Rev Biophys. Aug. 2003;36(3):307-40. Review.
Kaplan et al, Detachment of Actinobacillus actinomycetemcomitans biofilm cells by an endogenous beta-hexosaminidase activity. J Bacteriol. Aug. 2003;185(16):4693-8.
Grant et al, Synthetic Peptides for Production of Antibodies that Recognize Intact Proteins. In: Current Protocols in Molecular Biology Print Publication Date: Jul. 2002, pp. 11.16.1-19.
Hornbeck et al, Enzyme-Linked Immunosorbent Assays (ELISA). In: Current Protocols in Molecular Biology Print Publication Date: Jul. 1991, pp. 11.2.1-22.
GenEmbl database Acc. No. AY228551 from Kaplan et al, Detachment of Actinobacillus actinomycetemcomitans biofilm cells by an endogenous beta-hexosaminidase activity. J Bacteriol. Aug. 2003;185(16):4693-8. Alignment with SEQ ID No. 1.
Donlan, “Biofilms: Microbial Life on Surfaces”, Emerging Infectious Diseases, 8(9):881-890 (2002).
Database EMBL, “Mus musculus BAC clone RP24-363G13 from chromosome 3, complete sequence”, Accession No. AC127237 (Jul. 18, 2002).
Database EMBL, “Streptococcus suis FlpS (flpS) gene, complete cds; arginine deiminase operon operon, complete sequence; ArcD (arcD), ArcT (arcT), ArcH (arcH), and ArgR (argR) genes, compete cds; and unknown genes”, Accession No. AF546864 (Dec. 4, 2002).
Kaplan et al., 2001,Infect. Immun., 69:5375-5384 “Structural and genetic analyses of O polysaccharide fromActinobacillus actinomycetemcomitansserotype f”.
Kaplan et al., 2003,Bacteriology, 185(16):4693-4698 “Detachment ofActinobacillus actinomycetemcomitansBiofilm Cells by an Endogenous β-Hexosaminidase Activity”.
Kaplan et al., 2003,J. Bacteriol., 185(4):1399-1404 “Biofilm growth and detachment ofActinobacillus actinomycetemcomitans”.
Somerville et al., 1993,Proc. Natl. Acad. Sci. USA, 90:6751-6755 “Sequence analysis of the β-N-acetylglucosaminidase gene ofVibrio vulnificus: Evidence for a common evolutionary origin of hexosaminidases”.
Kaplan et al., 2002, “Biofilm dispersal ofNeisseria subflavaand other phylogenetically diverse oral bacteria,”Appl. Environ. Microbiol. 68(10): 4943-4950.
Kaplan, J.B., et al., “Actinobacillus actinomycetemcomitansDspB (dspB) gene, partial cds,” <http://www.ncbi.nlm.nih.gov/sviewer/viewer.fcgi?30420959:NCBI:5172251, Database GenBank [online], Accession No. AY228551, uploaded Aug. 4, 2003.
Clarke et al., “Cloning and Expression of the β-N-Acetylglucosaminidase Gene fromStreptococcus pneumoniae,” J. Biol. Chem. 270: 8805-8814 (1995).
Graham et al., “Molecular Cloning of the cDNA Which Encodes β-N-Acetylhexosaminidase A fromDictyostelium discoideum,” J. Biol. Chem. 263: 16823-16829 (1988).
Restriction Requirement of co-pending U.S. Appl. No. 11/938,617 (Jun. 14, 2010).
Dorn Brian R.
Merchant & Gould P.C.
Swope Sheridan
University of Medicine and Dentistry of New Jersey
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