Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2007-10-30
2007-10-30
Fredman, Jeffrey (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C536S023100, C435S287200
Reexamination Certificate
active
10287455
ABSTRACT:
The present invention is directed to a novel method of discriminating a highly infectious bacteriumBacillus anthracisfrom a group of closely related microorganisms. Sequence variations in the 16S and 23S rRNA of theB. cereussubgroup includingB. anthracisare utilized to construct an array that can detect these sequence variations through selective hybridizations. The identification and analysis of these sequence variations enables positive discrimination of isolates of theB. cereusgroup that includesB. anthracis. Discrimination of single base differences in rRNA was achieved with a microchip during analysis ofB. cereusgroup isolates from both single and in mixed probes, as well as identification of polymorphic sites. Successful use of a microchip to determine the appropriate subgroup classification using eight reference microorganisms from theB. cereusgroup as a study set, was demonstrated.
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Ha
Bavykin Sergei G.
Mirzabekov Andrei D.
Barnes & Thornburg LLP
Fredman Jeffrey
Martin Alice O.
The University of Chicago
Woolwine Samuel
LandOfFree
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