Discovery of and method for cloning and producing the PspGI rest

Details Discovery of and method for cloning and producing the PspGI rest Discovery of and method for cloning and producing the PspGI rest

1997-05-15

1998-12-15

Patterson, Jr., Charles L.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

43525233, 4353201, 536 232, C12N 1555, C12N 922

Patent

active

058495584

ABSTRACT:
The present invention relates to the type II restriction endonuclease PspGI, obtainable from Pyrococcus species G-I-H (NEB #906), an enzyme which recognizes the DNA sequence 5' CC(A/T)GG 3' and cleaves before the first C of the recognition sequence to produce a 5 base 5' extension:

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