Dirofilaria and onchocerca larval L3 cysteine protease proteins

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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435 711, 4352523, 4353201, 530324, 935 22, 935 66, C12P 2106, C12P 2104, A61K 3800, C12N 1500

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057926247

ABSTRACT:
The present invention provides for filariid nematode cysteine protease proteins; to filariid nematode cysteine protease nucleic acid molecules, in particular, Dirofilaria immitis L3 larval cysteine protease nucleic acid molecules and Onchocerca volvulus L3 larval cysteine protease nucleic acid molecules; to antibodies raised against such proteins, and to compounds that inhibit filariid nematode cysteine protease activity. The present invention also includes methods to obtain such proteins, nucleic acid molecules, antibodies and/or inhibitors. The present invention also includes therapeutic compositions comprising such proteins, nucleic acid molecules, antibodies and/or inhibitors, and the use of such compositions to protect an animal from disease caused by parasitic helminths.

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Molecular SIGMA Biology catalog, A New Dimension, 1989, Oligonucleotide Products, p. 54.
Abraham et al., "In Vitro Culture of Dirofilaria immitisThird-and Fourth-Stage larvae under Defined Conditions," The Journal of Parasitology, vol. 73, No. 2, pp. 377-383, Apr. 1987.
Richer et al., "Dirofilaria immitis: Proteases Produced by Third-and Fourth-Stage Larvae," Experimental Parasitology, vol. 75, pp. 213-222, 1992.
J. Sambrook e tal., Molecular Cloning A Laboratory Manual, Second Edition, Cold Spring Harbor Laboratory Press, pp. 9.47-9.51, 1989.

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