Directional cloning

Details Directional cloning Directional cloning

1989-03-07

1991-12-24

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435 91, 4353201, 935 18, 935 26, 935 27, 935 29, 935 31, 935 56, 935 58, C12N 1500, C12N 1510, C12N 1570, C12P 1934

Patent

active

050752278

ABSTRACT:
Methods for cloning cDNA and producing mRNA from the cloned cDNA are disclosed. The cDNA is cloned in one orientation through the use of a vector containing a directional cloning site and through the use of primer-adapter sequences complementary to portions of the directional cloning site. The vector may also contain a promoter, one or more terminators and a polyadenylation signal to facilitate transcription of the cloned cDNA and translation of the mRNA.

REFERENCES:
patent: 4342832 (1982-08-01), Goeddel et al.
Belyavsky, A. et al., 1989, Nucleic Acids Research, vol. 17, No. 8, pp. 2919-2932.
Belyaskii, A. V. et al., 1988, Dokl. Acad. Nauk SSSR, vol. 303, No. 6, pp. 1498-1501, and Chem. Abst. 1989, vol. 110, No. 17, p. 229, Abstr. 149153.
Sanger, F. et al., 1975, Journal of Molecular Biology, vol. 94, pp. 441-448.
Palazzolo, M. J. et al., 1987, Gene, vol. 52, pp. 197-206.
Promega Corporation, 1988/1989 Catalog and Applications Guide, 1988, Promega Corporation, Madison, Wisconsin, Section 12, p. 7.
Maniatis, T. et al., 1982, Molecular Cloning A Laboratory Manual, Cold Spring Harbor Laboratory, pp. 118, 119, 222.
Pharmacia Molecular Biologicals Catalog, 1986, Pharmacia, Piscataway, New Jersey, pp. 70, 72, 75, 82, 89.
Priess, H. et al.. 1980, Molecular and General Genetics, vol. 178, pp. 27-34.
Noma et al., Nature, 319:640-646, 1986.
Melton et al., Nuc. Acids Res., 12:7035-7056, 1984.
Dunn and Studier, J. Molec. Biol., 166:477-536, 1983.

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