Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase
Reexamination Certificate
2006-08-29
2006-08-29
Achutamurthy, P. (Department: 1652)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Oxidoreductase
C435S004000, C435S006120, C435S069100, C435S071100, C435S440000, C435S252300, C435S320100, C536S023200, C536S023700
Reexamination Certificate
active
07098010
ABSTRACT:
This invention relates to the expression of improved polynucleotide and polypeptide sequences encoding for eukaryotic enzymes, particularly oxidase enzymes. The enzymes are advantagoeusly produced in conventional or facile expression systems. Various methods for directed evolution of polynucleotide sequences can be used to obtain the improved sequences. The improved characteristics of the polypeptides or proteins generated in this manner include improved expression, enhanced activity toward one or more substrates, and increased thermal stability. In a particular embodiment, the invention relates to improved expression of the galactose oxidase gene and galactose oxidase enzymes. GAO mutants that are highly active and/or thermostable are disclosed.
REFERENCES:
patent: 5605793 (1997-02-01), Stemmer
patent: 5741691 (1998-04-01), Arnold et al.
patent: 5811238 (1998-09-01), Stemmer et al.
patent: 5830721 (1998-11-01), Stemmer et al.
patent: 5837458 (1998-11-01), Minshull et al.
patent: 5965408 (1999-10-01), Short
patent: 6090604 (2000-07-01), Golightly et al.
patent: 6498026 (2002-12-01), Delagrave et al.
patent: 752008 (1997-02-01), None
patent: 932670 (2000-08-01), None
patent: WO 95/22625 (1995-08-01), None
patent: WO 97/20078 (1997-06-01), None
patent: WO 97/35966 (1997-10-01), None
patent: WO 98/31837 (1998-07-01), None
patent: WO 98/41653 (1998-09-01), None
patent: WO 98/42832 (1998-10-01), None
patent: WO 00/00632 (2000-01-01), None
patent: WO 00/04190 (2000-01-01), None
patent: WO 00/09679 (2000-02-01), None
patent: WO 00/18906 (2000-04-01), None
patent: WO 01/62938 (2001-08-01), None
Rogers, M.S. et al.,Characterization of the Active Site of Galactose Oxidase and Its Active Site Mutational Variants Y495/F/H/K and W290H by Circular Dichroism Spectroscopy. Inorganica Chemica Acta.
Adanyi, N., et al. European Food Research and Technology, 1999; 209:220-226.
Aisaka, K., et al., Agric. Biol. Chem., 1981; 45(10):2311-2316.
Amaral, D., et al., Methods Enzymol., 1966; 9:87-92.
Anfinsen, C. B., Science, 1973; 181:223.
Arkin, A., et al., Proc. Natl. Acad. Sci. USA, 1992; 89:7811.
Arnold, F. H., Accounts Chem. Res., 1998; 31:125-131.
Arnold, F.H., et al., Adv. Biochem. Eng. Biotechnol., 1997;58:1-14.
Arnold. F.H. , FASEB J., 1993; 7:744-749.
Arts, S.J.H.F., et al., Synthesis-Stuttgart, 1997; 6:597-613.
Avigad, G., Arch. Biochem. Biophys., 1985; 239(2):531-537.
Avigad, G., Anal. Biochem., 1978;86:470-476.
Avigad, G., et al., J. Biol. Chem., 1962; 237;2736-2743.
Baron, A. J., et al., J. Biol. Chem., 1994; 269:25095-25105.
Beckman, R. A., et al., Biochemistry, 1994; 24:5810.
Bernarderz-Clark, E. D.; Georgiou, G. Inclusion Bodies and Recovery of Proteins from the Aggregated States. In Protein Refolding; Bernarderz-Clark, E. D., Georgiou, G., Eds.; ACS: Washington, D. C. p. 1-20 (1990).
Better, M., et al., Science, 1988; 240:1041.
Borman, C. D., et al., J. Biol. Inorg. Chem., 1997; 2:480-487.
Bradford, M., Anal. Biochem., 1978; 72:248-254.
Calderhead, D. M., et al., J. Biol. Chem., 1988; 263:12171-12174.
Caldwell, R. C.; Joyce, G. F. PCR Methods Applic. 2, 28(1992).
Calvin, N.M., et al , J. Bacteriol., 1988; 170(6):2796-2801.
Carbon, J., Clarke, L.; Ilgen, C.; Ratzkin, B. The Construction and Use of Hybrid Plasmid Gene Banks inEscherichia coli. In Recombinant Molecules: Impact on Science and Society; Beers, R. F. J., Bassett, E. G., Eds; Raven Press: New York, pp. 355-378 (1977).
Castelli, M. C. et al., Gene, 1994; 142 113.
Chang et al., Nature Biotechnol, 1999; 17:793-797.
Chen, K. & Arnold, F.H., Proc. Natl. Acad. Sci. USA, 1993; 90:5618-5622.
Cherry, J. R., et al., Nature Biotechnol., 1999; 17:379-384.
Christians et al., Nature Biotechnol., 1999; 17:259-264.
Cleland, J. L, et al., Bio/Technology, 1990; 8:1274.
Crameri, A , et al., Nature, 1998, 391:228-231.
Crameri, A. , et al., Nature Biotechnol., 1997; 15:436-438.
Crameri, A , et al., Nature Biotechnol., 1996; 14:315-318.
Crameri, A , et al., Nature Med., 1996; 2:100-103.
Crameri, A , et al., Angew. Chem. Int. Ed. Engl., 1980; 19:546-547.
De Sutter, et al., GENE, 1994; 141:163.
Delagrave et al., Bio/Technology, 1993; 11:1548.
Delagrave et al. Protein Engineering, 1993; 6:327.
Dower, W.J., et al , Nucleic Acids Res., 1988; 16(13):6127-6145.
Dunford, H.B., Peroxidases in Chemistry and Biology, 1991; vol. 2. pp. 1-24.
Egorov, A. M., et al., Ann. N. Y. Acad. Sci., 1991; 646:35.
Fiedler, K. & Simons, K., Cell, 1995; 81:309-312.
Fitzgerald et al., Biochemistry, 1994; 33:3807.
Gahmberg, C. G., and Tolvanen, M., Methods Enzymol., 1994; 230:32-44.
Gajhede, M., et al., Nature Struct. Biol., 1997; 4:1032.
Gazaryan, I.G., LABPV Newsletters, 1994; 4:8-15.
Gietz, D., et al., Yeast, 1995; 11 355.
Gillam, E.M., et al , Arch. Biochem. Biophys., 1995; 319:540-550.
Giver, L., et al., Proc. Natl. Acad. Sci. USA , 1998; 95:12809-12813.
Giver, L., and Arnold, F.H. Curr. Opinion Chem. Biol., 1998; 2: 335-338.
Goldman, E. R. and Youvan D. C., Bio/Technology, 1992; 10:1557.
Goodin, D. B., et al., Biochemistry, 1991; 30:4953.
Goshorn, S. C., et al., Cancer Res., 1993; 53:2123.
Gramm, H. et al., Proc. Natl. Acad. Sci. USA, 1992; 89:3576.
Guengerich et al., Meth. Enzymol., 1996; 272:35-44.
Gussow, D. & Clackson, T., Nucleic Acids Res., 1989; 17:4000-4000.
Hamilton et al., J. Am. Chem. Soc., 1978; 100(6):1899-1912.
Hamilton, G.A., de Jersey, J., and Adolf, P.K. (1973) Galactose oxidase : The complexities of a simple enzyme., in King, T.E., et al. Eds., Oxidases and related redox enzyme, University Park Press, Baltimore, MD, 103-124.
Haschke, R.H. & Friedhoff, J.M., Biochim. Biophys. Res. Commun., 1978; 80(4):1039-1042.
Helenius, A., Mol. Biol. Cell., 1994; 5:253-265.
Hermes, J. D. et al., Proc. Natl. Acad. Sci. USA, 1990; 87:696.
Ito et al., Methods Enzymol., 1995; 258:235-262.
Ito et al., J. Mol. Biol., 1994; 238:794-814.
Ito et al., Nature, 1991; 350:87-90.
Joo et al., Chem. Biol., 1999; 6:699-706
Joo et al., Nature, 1999; 399:670-673.
Khosla et al., Bio/Technology, 1990; 8:849-853.
Kiba et al., J. Chromatogr., 1989; 463:183-187.
Klibanov et al., Biochem. Biophys. Res. Commun., 1982; 108:804-808.
Knappik, A.; Pluckthun, A, Protein Eng., 1995; 8(1):81-89.
Koroleva et al., Prikl. Biokhim, Mikrobiol., 1983;19(5): 632-637.
Kosman, D.J. (1984) Galactose oxidase., in Lontie, R., Eds., Copper proteins and copper enzymes. vol. 2., CRC Press, Boca Raton, Fla., 1-26.
Koster et al., Synthesis, 1982; 650-652.
Kuchner, O., and Arnold, F.H., Trends Biotechnol., 1997; 15:523-530.
Lei et al., J. Bacteriol., 1987; 169:4379.
Liu et al., J. Am. Chem. Soc., 1999; 121:466-467.
Lis, M., and Kuramitsu, H.K. Antimicrob. Agents Chemother., 1997; 41(5):999-1003.
Leung, D. W. et al., Technique, 1989; 1(1):11-15.
Mannino et al., Italian Journal of Food Science, 1999; 11:57-65.
Maradufu et al., Carbohydr. Res., 1974; 32:93-99.
Maradufu et al., Canad. J. Chem., 1971; 49:3429-3436.
Marrs, B, L, IBC's Fifth Annual World Congress on Enzyme Technologies, Mar. 1, 2000.
Martin, I. G., Macias, E. M., Sanchez, J. S., and Rivera, B. G. (1998) Food Chemistry 61, 281-286.
Martin, B.D., Linhardt, R. J., and Dordick, J.S. (1998) Highly swelling hydrogels from ordered galactose-based polyacrylates., Biomaterials, 19(1-3), 69-76.
Martineau, P. et al., J. Mol. Biol., 1998; 20:117-127.
Mazur, A. W., and Hiler, G. D., J. Org. Chem., 1997; 62:4471-4475.
Mazur, A. in Enzymes in Carbohydrate Synthesis (1991) Bednarski, M. D. and Simon, E. S. Eds. pp. 99-110.
McPherson, M.J., Stevens, C., Baron, A.J., Ogel, Z.B., Seneviratne, K., Wilmot, C., Ito, N., Brocklebank, I., Phillips, S.E.V., and Knowles, P.F. (1993) Galactose oxidase: Molecular analysis and mutagenesis studies., Biochem. Soc. Transact., 21:752-756.
McPherson, M.J., et al. J. Biol. Chem., 1992; 267(12):8146-8152.
Mendonca, M.H., and Zancan, G.T., Arch. Biochem, Biophys., 1988; 266(2):427-434.
Mendonca, M. H., and Zancan, G. T. Arch. Biochem. Biophys., 1987; 252(2):507-514.
Miele, R.G., et al. J.Biol.Chem., 1999; 274 7769-7776
Minshull and Stemmer, Curr. Opin. Chem. Biol., 1999; 2:284-290.
Mitraki, A.; King, J. FEBS Lett., 1992; 307(
Arnold Frances H.
Petrounia Ioanna P.
Sun Lianhong
Achutamurthy P.
Buchanan Ingersoll LLP
California Institute of Technology
Pak Yong D.
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