Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1991-03-01
1993-06-29
Hill, Jr., Robert J.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 691, 4351723, 4352523, 4353201, 5303803, 5303875, C12N 1509, C12N 1562, C12N 1563
Patent
active
052234096
ABSTRACT:
In order to obtain a novel binding protein against a chosen target, DNA molecules, each encoding a protein comprising one of a family of similar potential binding domains and a structural signal calling for the display of the protein on the outer surface of a chosen bacterial cell, bacterial spore or phage (genetic package) are introduced into a genetic package. The protein is expressed and the potential binding domain is displayed on the outer surface of the package. The cells or viruses bearing the binding domains which recognize the target molecule are isolated and amplified. The successful binding domains are then characterized. One or more of these successful binding domains is used as a model for the design of a new family of potential binding domains, and the process is repeated until a novel binding domain having a desired affinity for the target molecule is obtained. In one embodiment, the first family of potential binding domains is related to bovine pancreatic trypsin inhibitor, the genetic package is M13 phage, and the protein includes the outer surface transport signal of the M13 gene III protein.
REFERENCES:
patent: 4304863 (1981-03-01), Collins et al.
patent: 4332897 (1982-12-01), Nakano et al.
patent: 4333397 (1982-11-01), Gilbert et al.
patent: 4348477 (1982-04-01), Nakano et al.
patent: 4348478 (1982-08-01), Nakano et al.
patent: 4411994 (1983-09-01), Gilbert et al.
patent: 4508826 (1985-11-01), Foor et al.
patent: 4593002 (1986-06-01), Palgecco
patent: 4595658 (1986-12-01), Zinder et al.
patent: 4595674 (1986-06-01), Tschesche et al.
patent: 4603112 (1986-08-01), Paolitti et al.
patent: 4642334 (1987-04-01), Moore et al.
patent: 4683202 (1987-07-01), Mullis
patent: 4703004 (1987-03-01), Hopp et al.
patent: 4704692 (1987-02-01), Ladner
patent: 4829052 (1989-05-01), Glover et al.
patent: 4894436 (1990-01-01), Auerswald et al.
patent: 4908773 (1990-03-01), Pantoliano et al.
patent: 5010175 (1991-04-01), Rutter et al.
Valenzuela, Pablo et al, "Antigen Engineering in Yeast: Synthesis and Assembly of Hybrid Hepatitis B Surface Antigen-Herpes Simplex 1 gD Particles," Bio/Technology, vol. 3, pp. 323-326, Apr. 1985.
CHAR88b: Charbit, et al.; Versatility of a vector for expressing foreign polypeptides at the surface of gram-negative bacteria; Gene (1988); 70(1):181-189.
FELI91: Felici, Franco; Selection of antibody ligands from a large library of oligopeptide expressed on a multivalent exposition vector; J. Mol. Biol. (1991); 222:301-310.
HEIJ90: von Heijne, et al.; Membrane proteins: from sequence to structure; Protein Engineering; (1990) 4(2):109-112.
ABAR84: Abarzua, P., Marians, K. J. "Enzymatic techniques for the isolation of random single-based substitutions in vitro at high frequency", PNAS (1984), 81:2030-2034.
AGTE87: Agterberg, et al., "Use of Outer Membrane Protein PhoE as a Carrier for the Transport of a foreign Antigenic Determinant to the Cell Surface of E. coli K-12"; Gene, 59 (1987) 145-50.
ALTM91: Altman, J. D., Henner, D. Nilsson, Anderson, S. Kunts, I. D. "Intracellular expression of BPTI fusion proteins and single column cleavage/affinity purification by chymotrypsin", Protein Engineering, (1991), 4(5):593-600.
ARGO87: Argos, P, "Analysis of Sequence-similar Pentapeptides in Unrelated Protein Teritary Structures", J Mol Biol (1987), 197:331-348.
ARAK90: Araki, K., M. Kuada, O. Ito, J. Kuroki, and S. Tachibana, "Four disulfide bonds allocation of Na.sup.+, K.sup.+ -ATPase inhibitor (SPAI)", Biochem Biophys Res Comm (1990), 172(1) 42-46.
AUSU87: Ausbel, F. M., R. Brent, R. E. Kingstong, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl, Editors Current Protocols in Molecular Biology, pp. 8.0.1-8.3.6, Greene Publishing Associates and Wiley-Interscience, Publishers: John Wiley & Sons, New York, 1987.
BARB91: Barbas, et al. "Assembly of combinatorial antibody libraries on phage surfaces: The gene III site", PNAS (1991) 88:7978-7982.
BASS90: Bass, et al. "Hormone phage:An enrichment method for variant proteins with altered binding properties", Proteins: Structure, Function, and Genetics (1990) 8:309-14.
BECK83: Beckwith, J. and T. J. Silhavy, "Genetic Analysis of Protein Export in Escherichia coli", Methods in Enzymology (1983), 97:3-11.
BECK89c: Becker, S., E. Atherton, and R. D. Gordon, "Synthesis and characterization of mu-conotoxin IIIa", Eur J Biochem, (Oct. 20, 1989), 185(1) 79-84.
BENS84: Benson, S. A., E. Bremer, and T. J. Silhavy, "Intragenic regions required for LamB export", Proc Natl Acad Sci USA (1984), 81:3830-34.
BENS87b: Benson, S. A., and E. Bremer, "In vivo selection and characterization of internal deletions in the lamB::lacZ gene fusion", Gene (1987), 52(2-3)165-73.
BENS87c: Benson, S. A., M. N. Hall, and B. A. Rasmussen, "Signal Sequence Mutations That Alter Coupling of Secretion and Translation of an Escherichia coli Outer Membrane Protein", J Bacteriol (1987), 169(10) 4686-91.
BENZ88b: Benz, R., "Structure and Function of Porins from Gram-Negative Bacteria", Ann Rev Microbiol (1988), 42:359-93.
BERG88: Berg, J. M., "Proposed structure for the zinc-binding domains from transcription factor IIIA and related proteins", Proc Natl Acad Sci USA (1988), 85:99-102.
BHAT86: Bhatnagar, P. K., and J. C. Frantz, "Synthesis and Antigenic activity of E. coli ST and its analogues", Develop biol Standard (1986), 63:79-87.
BOEK80: Boeke, J. D., M. Russel, and P. Model, "Processing of Filamentous Phage Pre-coat Protein: Effect of Sequence Variations near the Signal Peptidase Cleavage Site", J Mol Biol (1980), 144:103-116.
BOLI85: Bolin, In, Portnoy, D. A., Wolf-Watz, H, "Expression of the temperature-inducivle outer membrane Proteins of Yersiniae" Infection and Immunity (1985), 48(1):234-240.
BOTS85: Botstein, D. and D. Shortle, "Strategies and applications of in vitro mutagenesis", Science, (1985), 229(4719)1193-201.
BREI91: Breitling, et al. "A surface expression vector for antibody screening", Gene (1991), 104:147-153.
BRIN90: Brinkman, et al. "Design of an Aprotinin variant with inhibitory activity against Chymotrypsin and Cathepsin G by recombinant DNA technology", Biol. Chem. Hoppe-Seyler (1990), 371:43-52.
BURT91: Burton, et al. "A large array of human monoclonal antibodies to type 1 human immunodeficiency virus from combinatorial libraries of asymptomatic seropositive individuals", PNAS (1991) 88:10134-37.
BUTL85: Butler, C. A., Street, E. D., Hatch, T. P., Hoffman, P. S. "Disulfide-Bonded outer membrane proteins in the genus Legionella", Infection and Immunity, (1985), 48(1):14-18.
CHAN91: Chang, et al. "Expression of antibody Fab domains on bacteriophage surfaces potential use for antibody selection", J. of Immunol. (1991) 147:3610-14.
CHAR86b: Charbit, A., J. C. Boulain, and M. Hofnung, "Une methode genetique pur exposer un epitope choisi a la surface de la bacteria Escherichia coli. Perspectives [A genetic method to expose a chosen epitope on the surface of the bacteria E. coli]", Comptes Rendu Acad Sci, Paris, (1986), 302:617-24.
CHAR88b: Charbit, A., A. Molla, W. Saurin, and M. Hofnung, "Versatility of a vector for expressing foreign polypeptides at the surface of gram-negative bacteria", Gene (1988), 70(1)181-9.
CHAR88c: Charbit, A., S. Van der Werf, V. Mimic, J. C. Boulain, M. Girard, and M Hofnung, "Expression of a poliovirus neutralization epitope at the surface of recombinant bacteria: first immunization results", Ann Inst Pasteur Microbiol (1988), 139(1) 45-58.
CHAR90: Charbit, A., A. Molla, J. Ronco, J. M. Clement, V. Favier, E. M. Bahraoui, L. Montagnier, A. Leguern, and M. Hofnung, "Immunogenicity and antigenicity of conserved peptides from the envelope of HIV-1 expressed at the surface of recombinant bacteria", AIDS (1990), 4(6)545-51.
CHAV88: Chavrier, P., P. Lemaire, O. Revelant, R. Bravo, and P. Charnay, "Characterization of a Mouse Multigene Family That Encodes Zinc Finger Structures", Molec Cell Biol (1988), 8(3)1319-26.
CHOW87: Chowdhurry, K., U. Deutsch, and P. Gruss, "A Multigene Family Encoding Several `Finger` Structures Is Present and Differentially Active in Mammalian Genomes", Cell (1987), 48:771-778.
CLAC9
Guterman Sonia K.
Kent Rachel B.
Ladner Robert C.
Ley Arthur C.
Markland William
Cooper Iver P.
Hill Jr. Robert J.
Protein Engineering Corp.
Ulm John D.
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