Combinatorial chemistry technology: method – library – apparatus – Library – per se – Library contained in or displayed by a micro-organism or...
Reexamination Certificate
2002-07-31
2008-08-19
Schultz, J. Douglas (Department: 1639)
Combinatorial chemistry technology: method, library, apparatus
Library, per se
Library contained in or displayed by a micro-organism or...
C530S300000, C530S324000, C530S328000, C530S329000, C530S330000, C435S005000, C506S013000
Reexamination Certificate
active
07413537
ABSTRACT:
The invention relates, in part, to a library of chimeric proteins, each chimeric protein including a mini-protein between about eight and about forty amino acids long, wherein the mini-protein has a single disulfide bond formed by a pair of invariant cysteines and has only two cysteines. The chimeric protein also includes at least a portion of an outer surface protein of a genetic package, wherein the chimeric protein is displayed on the outer surface of the genetic package. The invention also includes, in part, a mixture of nucleic acids that encode a library of the invention. The invention also includes, in part, a process for identifying proteins with a desired binding activity against a target, the process including screening a library of chimeric proteins of the invention; and identifying the chimeric protein. The invention, in part, also includes chimeric proteins expressed by a library of the invention.
REFERENCES:
patent: 4304863 (1981-12-01), Collins et al.
patent: 4332897 (1982-06-01), Nakano et al.
patent: 4338397 (1982-07-01), Gilbert et al.
patent: 4348477 (1982-09-01), Nakano et al.
patent: 4348478 (1982-09-01), Nakano et al.
patent: 4359535 (1982-11-01), Pieczenik
patent: 4411994 (1983-10-01), Gilbert et al.
patent: 4508826 (1985-04-01), Foor et al.
patent: 4528266 (1985-07-01), Pieczenik
patent: 4593002 (1986-06-01), Dulbecco
patent: 4595658 (1986-06-01), Zinder et al.
patent: 4595674 (1986-06-01), Tschesche et al.
patent: 4603112 (1986-07-01), Paoletti et al.
patent: 4642334 (1987-02-01), Moore et al.
patent: 4683202 (1987-07-01), Mullis
patent: 4703004 (1987-10-01), Hopp et al.
patent: 4704692 (1987-11-01), Ladner
patent: 4757013 (1988-07-01), Inouye et al.
patent: 4769326 (1988-09-01), Rutter
patent: 4769327 (1988-09-01), Stephens et al.
patent: 4774180 (1988-09-01), Toth et al.
patent: 4786719 (1988-11-01), Kudo et al.
patent: 4797363 (1989-01-01), Teodorescu et al.
patent: 4829052 (1989-05-01), Glover et al.
patent: 4894436 (1990-01-01), Auerswald et al.
patent: 4908773 (1990-03-01), Pantoliano et al.
patent: 5010175 (1991-04-01), Rutter et al.
patent: 5101024 (1992-03-01), Kudo et al.
patent: 5223409 (1993-06-01), Ladner et al.
patent: 5225539 (1993-07-01), Winter
patent: 5403484 (1995-04-01), Ladner et al.
patent: 5432018 (1995-07-01), Dower et al.
patent: 5432155 (1995-07-01), Olivera et al.
patent: 5498538 (1996-03-01), Kay et al.
patent: 5571698 (1996-11-01), Ladner et al.
patent: 5616463 (1997-04-01), Fornace et al.
patent: 5625033 (1997-04-01), Kay et al.
patent: 5723286 (1998-03-01), Dower et al.
patent: 5747334 (1998-05-01), Kay et al.
patent: 5789538 (1998-08-01), Rebar et al.
patent: 5837500 (1998-11-01), Ladner et al.
patent: 5844076 (1998-12-01), Kay et al.
patent: 5852167 (1998-12-01), Kay et al.
patent: 5866363 (1999-02-01), Pieczenik
patent: 5891640 (1999-04-01), DeLeys
patent: 5948635 (1999-09-01), Kay et al.
patent: 5955582 (1999-09-01), Newman et al.
patent: 5969108 (1999-10-01), McCafferty et al.
patent: 6291158 (2001-09-01), Winter et al.
patent: 6979538 (2005-12-01), Ladner et al.
patent: 2002/0150881 (2002-10-01), Ladner et al.
patent: 2003/0113717 (2003-06-01), Ladner et al.
patent: 2003/0219886 (2003-11-01), Ladner et al.
patent: 2004/0005539 (2004-01-01), Ladner et al.
patent: 2004/0023205 (2004-02-01), Ladner et al.
patent: 0 184 187 (1986-06-01), None
patent: 297362 (1988-06-01), None
patent: 0 274 394 (1988-07-01), None
patent: 0 285 123 (1988-10-01), None
patent: 0 286 239 (1988-10-01), None
patent: 0285123 (1988-10-01), None
patent: 0286239 (1988-10-01), None
patent: 0 297 362 (1989-01-01), None
patent: 0 339 942 (1989-11-01), None
patent: 0 341 444 (1989-11-01), None
patent: 339942 (1989-11-01), None
patent: 341444 (1989-11-01), None
patent: 1 279 731 (1991-03-01), None
patent: 07010609.1 (2007-08-01), None
patent: 2 183 661 (1987-06-01), None
patent: 2 188 322 (1987-09-01), None
patent: 2188322 (1987-09-01), None
patent: 2 188 933 (1987-10-01), None
patent: 2188933 (1987-10-01), None
patent: 2208511 (1989-04-01), None
patent: 2 208 511 (1989-05-01), None
patent: WO 87/01374 (1987-03-01), None
patent: WO8701374 (1987-03-01), None
patent: WO 88/01649 (1988-03-01), None
patent: WO8801649 (1988-03-01), None
patent: WO 88/06601 (1988-09-01), None
patent: WO 88/06630 (1988-09-01), None
patent: WO8806601 (1988-09-01), None
patent: WO8806630 (1988-09-01), None
patent: WO 89/01968 (1989-03-01), None
patent: WO 89/02461 (1989-03-01), None
patent: WO8901968 (1989-03-01), None
patent: WO 90/02809 (1990-03-01), None
patent: WO9002809 (1990-03-01), None
patent: WO 91/17271 (1991-11-01), None
patent: WO9117271 (1991-11-01), None
patent: 91/18980 (1991-12-01), None
patent: 91/19818 (1991-12-01), None
patent: WO 91/18980 (1991-12-01), None
patent: WO 91/19818 (1991-12-01), None
patent: WO9118980 (1991-12-01), None
patent: WO 92/01047 (1992-01-01), None
patent: WO 92/06176 (1992-04-01), None
patent: WO 92/06204 (1992-04-01), None
patent: WO9206176 (1992-04-01), None
patent: WO9206204 (1992-04-01), None
patent: WO 92/11272 (1992-07-01), None
patent: WO9211272 (1992-07-01), None
patent: WO 92/15605 (1992-09-01), None
patent: WO 92/15677 (1992-09-01), None
patent: WO 94/18220 (1994-08-01), None
patent: WO 95/19431 (1995-07-01), None
patent: WO 96/06166 (1996-02-01), None
Huber et al . Biochemistry 18(19): 4090-4095 Interaction of divalent cations with beta-galactosidase, 1979.
Smith, G. P. Science 248(4705) 1315-137 Filamentous phage : novel expression vectors that display cloned antiges on the viron surface, Jun. 14, 1985.
Huse et al Science 246(4935):1275-1281. Generation of a large combinatorial library of immunoglobin repertoire in phage lambda, Dec. 8, 1989.
Iverson et al Science 243:1184-1188. Sequence specific peptide cleavage catalyzed by an antibody, Mar. 3, 1989.
Parmley et al. Gene 73: 305-318. Antibody-selectable filamentous fd phage vectors: affinity purification of target genes, 1988.
Rebar et al Phage display methods for selecting zinc finger proteins with novel DNA-binding specificities. Methods in Enzymology. vol. 267, pp. 129-149, 1996.
Choo et al Designing DNA-binding proteins on the surface of filamentous phage. Current Opnion in Biotechnology vol. 6., No. 4, 1995.
Fijima et al. Mar. 1986 Agric. Biol. Chem 50(3) 589-592.
Ferenci T. 1984 Trends in Biol Sci. 9 : 44-48.
Valenzuela, Pablo et al, “Antigen Engineering in Yeast: Synthesis and Assembly of Hybrid Hepatitis B Surface Antigen-Herpes Simplex 1 gD Particles,”Bio/Technology, vol. 3, pp. 323-326, Apr. 1985.
Charbit, et al.; Versatility of a vector for expressing foreign polypeptides at the surface of gram-negative bacteria; Gene (1988); 70(1):181-9.
Felici, Franco; Selection of antibody ligands from a large library of oligopeptide expressed on a multivalent exposition vector; J. Mol. Biol. (1991); 222:301-310.
von Heijne, et al.; Membrane proteins: from sequence to structure; Protein Engineering; (1990) 4(2):109-112.
Abarzua, P, Marians, K.J. “Enzymatic techniques for the isolation of random single-based substitutions in vitro at high frequency”, PNAS (1984), 81:2030-34.
Agterberg, et al., “Use of Outer Membrane Protein PhoE as a Carrier for the Transport of a foreign Antigenic Determinant to the Cell Surface ofE. coliK-12”; Gene, 59 (1987)145-50.
Altman, J.D., Henner, D, Nilsson, Anderson, S, Kunts, I.D. “Intracellular expression of BPTI fusion proteins and single column cleavage/affinity purification by chymotrypsin”, Protein Engineering, (1991), 4(5):593-600.
Argos, P, “Analysis of Sequence-similar Pentapeptides in Unrelated Protein Tertiary Structures”, J Mol Biol (1987), 197:331-348.
Araki, K, M Kuwada, O Ito, J Kuroki, and S Tachibana, “Four disulfide bonds allocation of Na+, K+-ATPase inhibitor (SPAI)”, Biochem Biophys Res Comm (1990), 172(1)42-46.
Ausubel, FM, R Brent, RE Kingston, DD Moore, JG Seidman, JA Smith, and K Struhl, EditorsCurrent Protocols in Molecular Biology, pp. 8.0.1-8.3.6, Greene Publishing Associates and Wiley-Interscience, Publishers: John Wiley & Sons, New
Guterman Sonia Kosow
Kent Rachel Baribault
Ladner Robert Charles
Ley Arthur Charles
Markland William
Dyax Corp.
Lundgren Jeffrey S.
Schultz J. Douglas
Wolf Greenfield and Sacks, P.C.
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