Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Method of regulating cell metabolism or physiology
Reexamination Certificate
2006-05-16
2006-05-16
Crouch, Deborah (Department: 1632)
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Method of regulating cell metabolism or physiology
C435S366000, C435S383000, C435S384000
Reexamination Certificate
active
07045353
ABSTRACT:
Methods are described for mapping a pathway of differentiation of a population of embryonic cells which includes exposing the cells to an exogenous factor and measuring gene expression products that are characteristic of a particular cell type or lineage. Directing differentiation of human embryonic cells relies on dissociated embryoid bodies which are then exposed to one or more exogenous factors to enrich a culture for a particular cell type. The differentiated cells may be used for treating a medical condition in a human. Kits for determining differentiation pathways and screening exogenous factors for their utility in differentiation are provided.
REFERENCES:
patent: 5453357 (1995-09-01), Hogan
patent: 5459069 (1995-10-01), Palsson et al.
patent: 5736396 (1998-04-01), Bruder et al.
patent: 5753506 (1998-05-01), Johe
patent: 5843780 (1998-12-01), Thomson
patent: 5874301 (1999-02-01), Keller et al.
patent: 5914268 (1999-06-01), Keller et al.
patent: 6090622 (2000-07-01), Gearhart et al.
patent: 6114168 (2000-09-01), Samarut et al.
patent: 6197585 (2001-03-01), Stringer
patent: 6602711 (2003-08-01), Thomson et al.
patent: 0 753 574 (1997-01-01), None
patent: 1 016 413 (2000-07-01), None
patent: WO 91/04274 (1991-04-01), None
patent: WO 96/15259 (1996-05-01), None
patent: WO 96/15259 (1996-05-01), None
patent: WO 96/17627 (1996-06-01), None
patent: WO 98/30679 (1998-07-01), None
patent: WO 98/43679 (1998-08-01), None
patent: WO 99/20740 (1999-04-01), None
patent: WO 99/38008 (1999-07-01), None
patent: WO 00/12683 (2000-03-01), None
patent: WO 0017326 (2000-03-01), None
patent: WO 00/70021 (2000-11-01), None
patent: WO 01/05944 (2001-01-01), None
Thomson et al. Embryonic Stem Cell Lines Derived from Human Blastocyts. Science. Nov. 6, 1998, vol. 282, pp. 1145-1147.
Keller. In Vitro Differentiation of Embryonic Stem Cells. Current Opinion in Cell Biology. 1995, vol. 7, pp. 862-869.
Wobus et al. Effects of Growth Factors on Differentiation Capacity of Embryonic Stem Cells In Vitro. Cell Different., vol. 20 (Suppl.) 81S.
Drab et al. From Totipotent Embryonic Stem Cells to Spontaneously Contracting Smooth Muscle Cells: A Retenoic Acid and db-cAMP In Vitro Differentiation Model. FASEB. 1997, vol. 11, pp. 905-915.
Vittet et al. Embryonic Stem Cells Differentiate In Vitro to Endothelial Cells Through Successive Maturation Steps. Blood. Nov. 1, 1996, vol. 88, pp. 3424-3431.
Thomson et al. Embryonic Stem Cell Lines Derived from Human Blastocysts. Science. vol. 282, Nov. 6, 1998, pp. 1145-1147.
Shamblott et al. Derivation of Pluripotent Stem Cells from Cultured Human Primordial Germ Cells. Proc. Natl. Acad. Sci. (USA). vol. 95, Nov. 1998, pp. 13726-13731.
Bain et al. Embryonic Stem Cells Express Neuronal Properties In Vitro. Developmental Bio. vol. 168, 1995, pp. 342-357.
Yuen et al. Generation of a Primative Erythroid Cell Line and Promotion of its Growth by Basic Fibroblast Growth Factor. Blood. vol. 91, May 1, 1998, pp. 3202-3209.
Wobus et al. Specific Effects of Nerve Growth Factor on the Differentiation Pattern of Mouse Embryonic Stem Cells In Vitro. Biomed. Biochim. Acta. vol. 47, 1988, pp. 965-973.
Effects of eight growth factors on the differentiation of cells derived from human embryonic stem cells, PNAS, XP-002198709, vol. 97, No. 21, pp. 11307-11312, Oct. 10, 2000.
Maintenance of pluripotential embryonic stem cells by stem cell selection, Reprod. Fertil. Devl. 1998, 10, 527-533P-001070570.
Retinoic Acid Accelerates Embryonic Stem Cell-Derived Cardiac Differentiation and Enhances Development of Centricular Cardiomyocytes, 1997 Academic Press Limited, J. Mol Cell Cardiol 29, 1525-1539 (1997), XP-002198708.
Single factors direct the differentiation of stem cells from the fetal and adult cental nervous system, Johe, Karl K., et al., Genes & Development, 10:3129-3140, 1996 by Cold Spring Harbor Laboratory Press ISSN 0890-9369/96, XP000867356.
Genetically Selected Cardiomyocytes from Differentiating Embryonic Stem Cells Form Stable Intracardiac Grafts, The American Society for Clinical Investigation, Inc. 0021-9738/96/07/216/09 vol. 98, No. 1, Jul. 1996, 216-224, XP000670716.
Expression of Transforming Growth Factor B2 during the Differentiation of Murine Embryonal Carcinoma and Embryonic Stem Cells, Academic Press, Inc., Developmental Biology 137, 161-170 (1990), XP-000891381.
Elsea et al. The Mousetrap:What We Can Learn When the Mouse Model Does Not Mimic the Human Disease, ILAR Journal, vol. 43, No. 2, pp. 66-79 (2002).
Thomson et al. Primate Embryonic Stem Cells, Curr. Top. in Dev. Biol., vol. 38, pp. 133-165 (1998).
Reubinoff, et al. “Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro,” Nature Biotechnology, vol. 18, No. 4, pp. 399-404, (2000).
Jung, et al. “Initiation of Mammalian liver Development from Endoderm by Fibroblast Growth Factors,” Science, vol. 284, pp. 1998-2003, (1999).
Itskovitz-Eldor, et al. “Differentiation of Human Embryonic Stem Cells into Embryoid Bodies Comprising the Three Embryonic Germ Layers,” Molecular Medicine, vol. 6, No. 2, pp. 88-95, (2000).
Thomson, et al. “Embryonic Stem Cell Lines Derived from Human Blastocysts,” Science, vol. 282, pp. 1145-1147, (1998).
Browdy and Neimark PLLC
Crouch Deborah
Yissum Research Development Company of The Hebrew University of
LandOfFree
Directed differentiation of human embryonic cells does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Directed differentiation of human embryonic cells, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Directed differentiation of human embryonic cells will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3648793