Direct radiolabeling of antibodies and other proteins with techn

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

530402, 424 11, A61K 39395

Patent

active

051029901

ABSTRACT:
Proteins containing one or more disulfide bonds are radiolabeled with radionuclides of technetium or rhenium for use in diagnosis and treatment of a variety of pathologic conditions. Radiolabeling is accomplished by partial reduction of the disulfide bonds of the protein using Sn (II), or using other reducing agents followed by the addition of Sn (II), removal of excess reducing agent and reduction by-products, and addition of a specified amount of pertechnetate or perrhenate reducing agent, such as stannous tartrate, with the addition accomplished in such manner that further reduction of the protein is limited. The resulting product may be stored frozen or lyophilized, with radiolabeling accomplished by the addition of pertechnetate or perrhenate solution.

REFERENCES:
patent: 4305922 (1981-12-01), Rhodes
patent: 4311688 (1982-01-01), Burchiel et al.
patent: 4323546 (1982-04-01), Crockford et al.
patent: 4331647 (1982-05-01), Goldenberg
patent: 4348376 (1982-09-01), Goldenberg
patent: 4421735 (1983-12-01), Haber et al.
patent: 4424200 (1984-01-01), Crockford et al.
patent: 4472371 (1984-09-01), Burchiel et al.
patent: 4478815 (1984-10-01), Burchiel et al.
patent: 4479930 (1984-10-01), Hnatowich
patent: 4647445 (1987-03-01), Lees
patent: 4652440 (1987-03-01), Paik et al.
patent: 4668503 (1987-05-01), Hnatowich
patent: 4670545 (1987-06-01), Fritzberg et al.
patent: 4877868 (1989-10-01), Reno
"Three Approaches to Radiolabeling Antibodies with 99mTc" by W. C. Eckelman et al., Nucl. Med Biol, vol. 16, No. 2, pp. 171-176 (1989).
"A New Method for Protein Labeling with 99mTc" by D. Blok et al., Nucl. Med Biol., vol. 16, No. 1, pp. 11-16 (1989).
"Covalent Attachment of Chelating Groups to Macromolecules" by G. E. Krejcarek et al., Biochemical and Biophysical Research Communications, vol. 77, pp. 581-585 (1977).
"`Bifunctional` Chelating Agents for Binding Metal Ions to Proteins" by T. G. Wensel et al., Radioimmunoimaging and Radioimmunotherapy, pp. 185-196 (1983).
"99m TC Labeling of Proteins: Initial Evaluation of a Novel Diaminedithiol Bifunctional Chelating Agent" by K. E. Baidoo, Cancer Research vol. 50, pp. 799-802 (1990).
"Radioimmunoimaging of Experimental Thrombi in Dogs Using Technetium-99-m Labeled Monoclonal Antibody Fragments Reactive with Human Platelets" by P. Som et al, Journal of Nuclear Medicine, vol. 27, No. 8, pp. 1315-1320 (1986).
"Technetium-99m--Albumin" by H. S. Stern et al., Book chapter published by Johns Hopkins Medical Institutions, Baltimore, Md., pp. 359-375 (U.S. Atomic Energy Commission) (1966).
"Use of Fe(II) or Sn(II) Alone for Technetium Labeling of Albumin" by M. S. Lin et al., Journal of Nuclear Medicine, vol. 12, pp. 204-211 (1971).
"99m Tc-Human Serum Albumin" by W. C. Eckelman et al., Journal of Nuclear Medicine, vol. 12, pp. 707-710 (1971).
"A Rapid Chemical Method of Labeling Human Plasma Proteins with 99m Tc-Pertechnetate at pH 7.4" by D. W. Wong et al., Journal of Applied Radiation and Isotopes, vol. 29, pp. 251-253.
"A Rapid Method for Labeling IgG with 99m" by L. G. Colombetti et al., Abstract from Journal of Nuclear Medicine, Proceedings of 26th Annual Meeting, p. 652 (1979).
"A Novel Approach to Tc-99m-Labeled Monoclonal Antibodies" by A. Schwarz et al., Proceedings of 34th Annual Meeting; vol. 28, No. 4, p. 721 (1987).
"A Rapid and Efficient Method for Labeling IgG Antibodies with Tc-99m and Comparison to Tc-99m FAB' Antibody Fragments" by K. Y. Pak et al., Abstract from Proceedings of the 36th Annual Meeting, vol. 30, No. 5, p. 793 (1989).
"Imaging of Inflammatory Arthritis with Technetium-99m-Labeled IgG" by F. Breedveld et al., Journal of Nuclear Medicine, pp. 2017-2021 (1989).
"Coupling of the 99m Technetium-Nitrido Group to Monoclonal Antibody and Use of the Complexes for the Detection of Tumors in Mice" by J. Kanellos, JNCI, vol. 77, No. 2, pp. 431-439 (1986).
"Technetium-99m Labeling of Murine Monoclonal Antibody Fragments" by Rhodes et al., Journal of Nuclear Medicine, vol. 27, No. 5 (1986), pp. 685-693.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Direct radiolabeling of antibodies and other proteins with techn does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Direct radiolabeling of antibodies and other proteins with techn, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Direct radiolabeling of antibodies and other proteins with techn will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-1896373

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.