Drug – bio-affecting and body treating compositions – Radionuclide or intended radionuclide containing; adjuvant... – Molecular bilayer structure
Patent
1974-06-17
1977-03-15
Padgett, Benjamin R.
Drug, bio-affecting and body treating compositions
Radionuclide or intended radionuclide containing; adjuvant...
Molecular bilayer structure
23230B, 424 12, 424 15, G01N 3300, G01N 3316, G21H 502
Patent
active
040124940
ABSTRACT:
A method for direct radioimmunoassay of antigens or their associated antibodies utilizing a coated test apparatus comprising adding a serum to be tested for an antigen to test apparatus coated with its antibody; incubating the tubes for from 0.5 to 42 hours; aspirating the contents and washing the same with a Tris-HCl and sodium azide mixture; adding purified I.sup.125 labeled antibody into the tube and incubating for from 1 to 24 hours; aspirating and washing the contents; and counting the tube for I.sup.125 radiation. A similar assay for the antibody may be conducted utilizing an antigen coated apparatus and I.sup.125 labeled antigen. The method for coating these apparatus such as tubes comprises adding the antigen or antibody solution in a Tris-HCl, sodium azide solution into a suitable test tube or well, incubating the tubes at room temperature, aspirating and washing the contents and storing at from between 2.degree. and 25.degree. C. until use.
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Haberman, Radioimmunoassay and Related Procedures in Medicine, vol. I, IAEA, Vienna, 1974, pp. 341-353.
Koutoulidis et al., Radioimmunoassay and Related Procedures in Medicine, vol. II, IAEA, Vienna, 1974, pp. 377-383.
Abbott Laboratories
Niblack Robert L.
Nucker Christine M.
Padgett Benjamin R.
Weist Robert D.
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