Direct radio-frequency detection of nucleotide hybridization...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C435S283100, C536S023100, C536S024300

Reexamination Certificate

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10694028

ABSTRACT:
Radio-frequency (RF) excitation is used for direct detection of hybridization events at microelectrodes with surface-attached DNA oligomers. A homodyne reflectometer operates on a high frequency carrier to detect the presence of a low-frequency modulation signal. Without non-linearities in an interface, the modulation signal is not impressed upon the carrier signal. As such, the reflectometer can sensitively measure changes in dielectric properties without interference from other sources of capacitance/resistance unrelated to the reaction at the surface.

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Souteyrand, et al., “Direct Detection of the Hybridization of Synthetic Homo-Oligomer DNA Sequences by Field Effect,”J Phys Chem B(101) pp. 2980-2985, 1997.
Berggren, et al, “A Feasibility Study of a Capacitive Biosensor for Direct Detection of DNA Hybridization,”Electroanalysis(11)3: pp. 156-160, 1999.
Souteyrand, et al., “Comparison Between Electrochemical and Optoelectrochemical Impedance Measurements for Detection of DNA Hybridization,”J Appl. Biochem Biotech(89) : pp. 195-207, 2000.
Berney, et al., “A DNA Diagnostic Biosensor: Development, Characterisation and Performance,”Sensors and Actuators B 68, pp. 100-108, 2000.
W. Cai, et al., Abstract for AVS 49th International Symposium, available on the worldwide web at www.avssymposium.org at least as early as Oct. 26, 2002.

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