Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1997-08-05
2000-01-11
Campbell, Eggerton A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 912, C12Q 168, C12P 1934
Patent
active
060134429
ABSTRACT:
A direct quantitation of RNA contained in a sample is obtained by capillary electrophoresis of the RNA hybridized to a DNA probe of complementary sequence stabilized by the combination of a fluorophore terminally conjugated to the DNA probe and a dye intercalating the RNA-DNA hybrid so formed. The RNA is quantified by measuring the total fluorescence emitted by the electrophoresed hybrid upon excitation by a laser generated light beam.
REFERENCES:
Database WPI, Section Ch, Week 9131, Derwent Publications Ltd., London, GB; Class B04, AN 91-227685, XP002084014 & JP 03 147797 A (Shimadzu Corp), Jun. 24, 1991, see abstract.
Fang C., et al., "Sequence-Dependent Separation of DNA Fragments by Capillary Electrophoresis in the Presence of SYBR.RTM. Green I", BioTechniques, vol. 23, Jul. 1997, pp. 58, 60.
Kolesar J.M., et al., "Direct quantification of HIV-1 RNA by capillary eletrophoresis with laser-induced fluorescence", Journal of Chromatography B, vol. 697, Sep. 12, 1997, pp. 189-194.
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Bianchi et al., Capillary Electrophoresis: Detection of Hybridization Between Synthetic Oligonucleotides and HIV-1 Gernomic DNA Amplified by Polymerase-Chain Reaction, J. Virol. Meth. 47:321-29 (1994).
Todd et al., Quantification of Human Immunodeficiency Virus Plasma RNA By Branched DNA and Reverse Transcription Coupled Polymerase Chain Reaction Assay methods; A Critical Evaluation of Accuracy and Reproducibility; Serodiagn. Immunother. Infect. Disease 6(4):233-39 (1994).
Fernandez-Arcas et al., Direct Quantification of HIV-1 RNA in Human Plasma by Free Solution Capillary Electrophoresis, J. Acq. Imm. Defic. Syn. Human Retrovir. 12:107-11 (1996).
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Allen Peter G.
Kolesar Jill M.
Campbell Eggerton A.
Welch Teresa J.
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