Direct quantification of gene expression using capillary...

Details Direct quantification of gene expression using capillary... Direct quantification of gene expression using capillary...

2011-05-10

2011-05-10

Dejong, Eric S (Department: 1631)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S283100, C702S019000

Reexamination Certificate

active

07939252

ABSTRACT:
Provided is a method for direct quantification of gene expression using capillary electrophoresis with laser-induced fluorescence to measure RNA in a sample. Also provided is a method of diagnosing a disease in a subject, wherein the disease is caused by increased or decreased expression of a causative gene.

REFERENCES:
Al-Mahrouki, et al., “Calibration-Free Quantitative Analysis Of mRNA,”Anal. Chem. 77: 8027-8030 (2005).
Bodkin, et al., “Assessment of Sequence Relatedness Of Double-Stranded RNA Genes by RNA-RNA Blot Hybridization,”J. Virol. Methods, 10: 45-52 (1985).
Casey, et al., “Rates Of Formation And Thermal Stabilities Of RNA:DNA And DNA:DNA Duplexes At High Concentrations Of Formamide,”Nucl. Acids Res. 4: 1539-1552 (1977).
Espy, et al., “Real-time PCR In Clinical Microbiology: Applications For Routine Laboratory Testing,”Clin. Microbiol. Rev. 19: 165-256 (2006).
Fasco, et al., “Competitive RNA-PCR by Capillary Electrophoresis and Laser-Induced Fluorescence (LIF) Detection for Quantitation of Cellular mRNA,” Beckman Application Information,Nucleic AcidsA1788A: 1-5 (1994).
Ferri, et al., “Role of Discoidin Domain Receptors 1 and 2 In Human Smooth Muscle Cell-Mediated Collagen Remodeling,”Am. J. Pathol. 164: 1575-1585 (2004).
Han, et al., “Monitoring Differential Synthesis Of RNA In Individual Cells By Capillary Electrophoresis,”Anal. Biochem. 302: 136-143 (2002).
Han, et al., “In situ Sampling And Separation Of RNA From Individual Mammalian Cells,”Anal. Chem. 72: 4073-4079 (2000).
Kolesar, et al., “Direct Quantification Of HIV-1 RNA By Capillary Electrophoresis With Laser-Induced Fluorescence,”J Chromatogr. B, 697: 189-194 (1997).
Lai, et al., “Structure And Expression Of The Tyro 10 Receptor Tyrosine Kinase,”Oncogene, 9: 877-883 (1994).
Odin, et al., “Rapid Method For Relative Gene Expression Determination In Human Tissues Using Automated Capillary Gel Electrophoresis And Multicolor Detection,”J. Chromatogr. B, 734: 47-53 (1999).
Richards, et al., “Analysis Of Leptin Gene Expression In Chickens Using Reverse Transcription Polymerase Chain Reaction And Capillary Electrophoresis With Laser-Induced Fluorescence Detection,”J. Chromatogr. A, 853: 321-335 (1999).
Sobczak, et al., “RNA Structure Analysis Assisted By Capillary Electrophoresis,”Nucl. Acids Res, 30: e124 (2002).
van Eekelen, et al., “Quantitative Analysis Of Cytokeratin 20 Gene Expression Using RT-PCR And Capillary Electrophoresis With Fluorescent DNA Detection,”Clin. Biochem, 33: 457-464 (2000).
Zabzdyr, et al., “A Qualitative Look At Multiplex Gene Expression Of Single Cells Using Capillary Electrophoresis,”Electrophoresis, 26: 137-145 (2005).

Affiliated with

Also associated with

Rating

Direct quantification of gene expression using capillary... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Direct quantification of gene expression using capillary..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Direct quantification of gene expression using capillary... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2660821