Direct molecular diagnosis of Friedreich ataxia

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 912, 435 9121, 435 915, 435 9151, 435810, 536 235, 536 2431, 536 2433, 935 8, 935 9, 935 77, 935 78, C12Q 168, C12P 1934, C07H 2104

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active

061500917

ABSTRACT:
This invention relates generally to methods for the diagnosis and therapeutic treatment of Friedreich Ataxia. Friedreich ataxia (FRDA) is an autosomal recessive, degenerative disease that involves the central and peripheral nervous system and the heart. A gene, X25, was identified in the critical region for the FRDA locus on chromosome 9q13. The gene encodes a 210 amino acid protein, frataxin, that has homologues in distant species such as C. elegans and yeast. A few FRDA patients have been found to have point mutations in X25, but the vast majority are homozygous for a variable, unstable GAA trinucleotide expansion in the first X25 intron. Mature X25 mRNA was severely reduced in abundance in individuals with FRDA. Carriers and individuals at risk for developing FRDA can be ascertained by the methods of the present invention. Further, the methods of the present invention provide treatment to those individuals having FRDA.

REFERENCES:
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Montermini, L. et al., The friedreich Ataxia Critical Region Spans a 150-kb Interval on Chromosome 9q13, Am. J. Hum. Genet. 57:1061-1067 (1995).
Duclos, F. et al., Gene in the Region of the Friedreich Ataxia Locus Encodes a Putative Transmembrane Protein Expressed in the Nervous System, Proc. Natl. Acad. Sci. USA, 90:109-113 (1993).
Gacy, A.M. et al., Trinucleotide Repeats That Expand in Human Disease Form Hairpin Structures in Vitro, Cell 81:533-540 (1995).
Campuzano, V. et al., Friedreich's Ataxia: Autosomal Recessive Disease Caused by an Intronic GAA Triplet Repeat Expansion, Science 271:1423-1427 (1996).
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