Direct labeling of antibodies and other protein with metal ions

Details Direct labeling of antibodies and other protein with metal ions Direct labeling of antibodies and other protein with metal ions

1992-01-03

1995-10-24

Geist, Gary

Drug, bio-affecting and body treating compositions

Radionuclide or intended radionuclide containing; adjuvant...

Attached to antibody or antibody fragment or immunoglobulin;...

5303913, 5303915, 530866, A61K 4902, A61K 4300, A61K 39395, C07B 5900

Patent

active

054607850

ABSTRACT:
Proteins containing one or more dtsulfide bonds or monosulfides are labeled with medically useful metal ions for use in diagnosis and treatment of a variety of pathologtc conditions. Labeling is accomplished with a variety of metals, including radiometals, by using a reducing agent to reduce the disulfide bonds to thiolate groups; excess reducing agent, reaction by-products and any impurities are removed; and, a source of Sn (II) agent is added to the thiolate-containing protein. The resulting product may be stored frozen or lyophilized, with labeling accomplished by the addition of the medically useful metal ions.

REFERENCES:
patent: 3812264 (1974-05-01), Nouel
patent: 4424200 (1984-01-01), Crockford et al.
patent: 4472371 (1984-09-01), Burchiel et al.
patent: 4478815 (1984-10-01), Burchiel et al.
patent: 4485101 (1984-11-01), Coy et al.
patent: 4853371 (1989-08-01), Coy et al.
patent: 4871717 (1989-10-01), Coy et al.
patent: 4877868 (1989-10-01), Reno et al.
patent: 4904642 (1990-02-01), Coy et al.
patent: 5011676 (1991-04-01), Thakur
patent: 5053493 (1991-10-01), Pak et al.
patent: 5061641 (1991-10-01), Shochat et al.
patent: 5078985 (1992-01-01), Rhodes
patent: 5102990 (1992-04-01), Rhodes
patent: 5116596 (1992-05-01), Bremer et al.
patent: 5128119 (1992-07-01), Griffiths
patent: 5225180 (1993-07-01), Dean et al.
patent: 5317091 (1994-05-01), Subramanian
patent: 5328679 (1994-07-01), Hansen et al.
Rhodes et al., J. Nucl. Med., vol. 27 (1986) "Techetium-99m Labeling . . . " pp. 685-693.
Albert, R., et al., "A ISomatostatin Analogue to Image SS-Receptor-Positive Tumours", 12th American Peptide Symposium, Abstract LM10 (1991).
Bakker, W. H., et al., "In Vivo Use of a Radiodinated Somatostatin Analog: Dynamics, Metabolism, and Binding to Somatostatin Receptor-Positive Tumors in Man," J. Nucl. Med., vol. 32, No. 6 (1991).
Bakker, W. H., et al., "Receptor Scintigraphy with a radioiodinated Somatostatin Analog: Radiolabeling, Purification, Biologic Activity, and In Vivo Application in Animals,"]J. Nucl. Med., vol. 31, No. 9, (Sep. 1990).
Kwekkeboom, D. J., ".vertline..sup.In-111-DTPA-D-Phe .vertline..sub.1 -Octreotide Scintigraphy in Neuro-Endocrine Tumors," The Journal of Nuclear Medicine, Proc. of 38th Ann. Meeting, vol. 32, No. 5, p. 981, (1991).
"The Labeling of High Affinity Sites of Antibodies with 99mTc," by Chang H. Paik et al., INt. J. Nucl. Med. Biol., vol. 12, No. 1, pp. 3-8 (1985).
Anderson, P., et al., "Antibodies Labeled with .sup.199 Au: Potential for .sup.199 Au: for Radioimmunotherapy," Nucl. Med. Biol., vol. 15, No. 3, pp. 293-297, (1988).
Deshpande, S. V., et al., "Copper-67-Labeled Monoclonal Antibody Lym-1, A Potential Radiopharmaceutical for Cancer Therapy: Labeling and Biodistribution in RAJI Tumored Mice," J. Nucl. Med., vol. 29, No. 2, pp. 217-225, (Feb. 1988).
DeNardo, G. L., et al., "Requirements for a Treatment Planning System for Radioimmunotherapy," Int. J. Radiol. Oncology Biol. Phys., vol. 11, pp. 335-348, (1985).
Esser, Peter D., Appendix, "Radioactive Tracers," Freeman & Johnsons Clinical Radionuclide Imaging, 3rd Ed., pp. 1491-1513 (1984).
Granowska, M., et al., "A Tc-99m Labelled Monoclonal Antibody, PR1A3, for Radioimmunoscintigraphy, RIS, of Colorectal Cancer," Proceedings of the 36th Annual Meeting, J. Nucl. Med., vol. 30, p. 748 (No. 80), (1989).
Green, M. A., et al., Review, "Gallium Radiopharmaceutical Chemistry," Nucl. Med. Biol., vol. 16, No. 5, pp. 435-448, (1989).
Hainfeld, James F., et al., "Radioactive Gold Cluster Immunoconjugates: Potential Agents for Cancer Therapy," Nucl. Med. Biol., vol. 17, No. 3, pp. 287-294, (1990).
Mercer-Smith, Janet A., et al., "The Biodistribution of Radiocopper-Labeled Compounds," Copper Bioavailability and Metabolism, C. Kies, Ed., pp. 103-121, (1990).
Pak, K. Y., et al., "A Rapid and Efficient Method for Labeling IgG Antibodies with Tc-99m and Comparison to Tc-99m FAB' Antibody Fragments," Scientific Papers, Proceedings of the 36th Annual Meeting, J. Nucl. Med., vol.l 30, No. 5, p. 793 (No. 268), (1989).
Pietersz, G. A., et al., Special Invited Article, "The Use of Monoclonal Antibody Conjugates for the Diagnosis and Treatment of Cancer," Immunology and Cell Biology, vol. 65, Pt. 2, pp. 111-125, (1987).
Rayudu, G. V. S., "Production of Radionuclides for Medicine," Sem. Nucl. Med., vol. 44, pp. 100-110, (1990).
Rhodes, Buck A., et al., "Technetium-99m Labeling of Murine Monoclonal Antibody Fragments," J. Nucl. Med., vol. 27, No. 6, pp. 685-693, (May 1986).
Roberts, Jeanette C., et al., "Preparation and Characterization of Copper-67 Porphyrin-Antibody Conjugates," J. Immunol. Meth., vol. 105, pp. 153-164, (1987).
Roberts, Jeanette C., et al., "Labeling Antibodies with Copper Radionuclides Using N-4-Nitrobenzyl-5-(4-carboxyphynyl)-10,15,20-tris(4-sulfophenyl)Porphine," Appl. Radiat. Isot., vol. 40, No. 9, pp. 775-781, (1989).
Som, P., et al., "Radioimmunoimaging of Experimental Thrombi in Dogs Using Technetium-99-m-Labeled Monoclonal Antibody Fragments Reactive with Human Platelets," J. Nucl. Med., vol. 27, No. 8, pp. 1315-1320, (Aug. 1986).
Schwarz, A., et al., "A Novel Approach to Tc-99-m-Labeled Monoclonal Antibodies," Poster Sessions, Proceedings of 34th Ann. Mtg., J. Nucl. Med., vol. 28, No. 4, p. 721 (No. 695), (Apr. 1987).

Affiliated with

Also associated with

Rating

Direct labeling of antibodies and other protein with metal ions does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Direct labeling of antibodies and other protein with metal ions, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Direct labeling of antibodies and other protein with metal ions will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1884787