Direct immunoassay using pH-sensitive fluorochromes

Details Direct immunoassay using pH-sensitive fluorochromes Direct immunoassay using pH-sensitive fluorochromes

1991-11-14

1994-08-02

Kepplinger, Esther L.

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

436536, 436546, 436800, G01N 33543, G01N 33536

Patent

active

053345371

ABSTRACT:
The complexing of an antibody-antigen binding pair is determined by observing the change in fluorescence of a pH-sensitive fluorochrome attached to one of the members of the binding pair. When the binding is conducted in a solution having a pH other than the isoelectric point of the antibody, there will be a change in the pH of the microenviromnent surrounding the fluorochrome. This change will correspond to a change in the observed fluorescent intensity. Either member of the binding pair can be labeled, and combined with that member whose presence is suspect, in an immunoassay.

REFERENCES:
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Ullman et al. JBC 251 #14 pp. 4172-4176 (1976).
Nargessi: et al. Methods of Enzymology 74 pp. 60-79 (1981).
Smith FEBS Letters 77 #1 pp. 25-27 (1977).
Meadows et al. J. Immunol. Meth. 143 pp. 263-272 (1991).

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