Direct gene transfer into the ruminant mammary gland

Details Direct gene transfer into the ruminant mammary gland Direct gene transfer into the ruminant mammary gland

1995-01-20

1998-07-14

Crouch, Deborah

Drug, bio-affecting and body treating compositions

In vivo diagnosis or in vivo testing

800 2, 800DIG2, 935 60, 4351723, 435 691, A61K 4900, C12N 1500, C12N 500, C12P 2106

Patent

active

057800090

ABSTRACT:
Disclosed is a method for expressing a heterologous gene in the mammary gland of a ruminant mammal. The invention is useful for producing a heterologous protein(s) in the milk of the mammal, for producing a heterologous enzyme(s) which is tethered to the mammary epithelial cell membrane and which can act on a component of the milk of the mammal, and for providing an antisense oligonucleotide(s) or ribozyme(s) which inhibits expression of a gene to a mammary epithelial cell. The method involves infusing a liquid complex including a genetic construct into a ductal tree of the mammal. The liquid complex can be infused before the mammal reaches sexual maturity and after the mammal develops a functional streak canal. Alternatively, a liquid complex that is free of live retroviruses can be infused. If desired, the infused genetic construct can be treated with a polycationic compound and/or a lipid to improve the efficiency with which it is taken up by an epithelial cell of the mammary gland.

REFERENCES:
patent: 4861718 (1989-08-01), Hirata et al.
patent: 4873316 (1989-10-01), Meade et al.
patent: 5047340 (1991-09-01), Dickson et al.
patent: 5215904 (1993-06-01), Gould et al.
patent: 5227301 (1993-07-01), Turner et al.
patent: 5304489 (1994-04-01), Rosen
patent: 5322775 (1994-06-01), Clark et al.
Hennighausen, L., "The Prospects for Domesticating Milk Protein Genes, " J. Cell. Bio. 49: 325-332 (1992).
Romagnolo, D. et al., "Transgenic Approaches for Modifying the Mammary Gland to Produce Therapeutic Proteins," Environmental Health Perspectives, 102:846-51 (1994).
Yom, Heng-Cherl et al., Am.J.Clin.Nutr., vol. 58(2) Suppl., pp. 299S-306S, 1993.
Archer et al., "Human Growth Hormone (hGH) Secretion in Milk of Goats After Direct Transfer of the hGH gene into the Mammary Gland by Using . . . ", Proc. Natl. Acad. Sci. USA, 91:6840:6844 (1994).
Brown et al., "Mechanism of Membrane Anchoring Affects Polarized Expression of Two Proteins in MDCK cells," Science, 245:1499-1501 (1989).
Cannino et al., "Gene Expression of a Thermostable .beta.-Galactosidase in Mammalian Cells and its Application in Assays of Eukaryotic Promoter Activity," Biotechnol. Appl. Biochem., 19:233-244 (1994).
Collier et al., "Effect of Reserpine on Milk Production and Serum Prolactin of Cows Hormonally Induced into Lactation," Journal of Dairy Science, 60:896-901 (1976).
DiTullio et al., "Production of Cystic Fibrosis Transmembrane Conductance Regulator in the Milk of Transgenic Mice," Bio/Technology, 10:74-77 (1992).
Dong et al., "Efficient DNA Transfection of Quiescent Mammalian Cells using Poly-L-Orthine," Nucleic Acids Research, 21:771-772 (1993).
Eckhardt et al., "Characterization of the Promoter, Signal Sequence, and Amino Terminus of a Secreted .beta.-Galactosidase from `Streptomyces Lividans`", Journal of Bacteriology, 169:4249-4256 (1987).
Gorodetsky et al., "Isolation and Characterization of the Bos tauras .beta.-casein Gene," Gene, 66:87-96 (1988).
Hubbes et al., "Human Placental .beta.-Galactosidase," Biochem. J., 285:827-831, (1992).
Janne et al., "Transgenic Bioreactors," Int. J. Biochem., 26:859-870 (1993).
Kalnis et al., "Sequence of the lacZ Gene of Escherichia Coli," The EMBO Journal, 2:593-597 (1983).
Kong et al., "Targeting of Recombinant Na+/glucose Cotransporter (SGLT1) to the Apical Membrane," FEBS, 333:1-4 (1993).
Kumar et al., "Saccharomyces Cerevisiae Cells Secreting an Aspergillus Niger .beta.-Galactosidase Grow on Whey Permeate," Bio/Technology, 10:82-85 (1992).
Lee et al., "Production of Biomedical Proteins in the Milk of Transgenic Dairy Cows; the State of the Art," Journal of Controlled Release, 29:213-221 (1994).
Mantei et al., "Complete Primary Structure of Human and Rabbit Lactase-Phlorizin Hydrolase: Implications for Biosynthesis, Membrane Anchoring and Evolution of the Enzyme," The EMBO Journal, 7:2705-2713 (1988).
Masibay et al., "Expression of Bovine .beta.-1,4-galactosyltransferase cDNA in COS-7 Cells," Proc. Natl. Acad. Sci. USA, 86:5733-5737 (1989).
Masri et al., "Identification of the Full-Length Coding Sequence for Human Galactosyltransferase (.beta.-N-Acetylglucosaminide: .beta.1,4-Galactosyltransferase)," Biochemical and Biophysical Res. Comm. 157:657-663 (1988).
O'Leary et al., "A Comparison of Some Chemical Properties of Yogurts Made from Control and Lactase-Treated Milks," Journal of Food Science, 41:791-793 (1976).
Oshima et al., "Cloning, Sequencing, and Expression of cDNA for Human .beta.-Galactosidase," Biochemical and Biophysical Res. Comm., 157:238-244 (1988).
Poch et al., "Sequence of the Kluyveromyces lactis .beta.-galactosidase: Comparison with Prokaryotic Enzymes and Secondary Structure Analysis," Gene, 118:55-63 (1992).
Rahim et al., "Production and Characterization of .beta.-Galactosidase from Psychrotrophic Bacillus subtilis KL88," Biotechnology and Applied Biochemistry, 13:246-256 (1991).
Schmidt et al., "Expression and Nucleotide Sequence of the Lactobacillus bulgaricus .beta.-Galactosidase Gene Cloned in Escherichia coli," Journal of Bacteriology, 171:625-635 (1989).
Schroeder et al., "Analysis of the lacZ sequences from two Streptococcus thermophilus strains: Comparison with Escherichia coli . . . ," Journal of General Microbiology, 137:369-380 (1991).
Shaper, et al. "Bovine Galactosyltransferase: Identification of a Clone by Direct Immunological Screening of a cDNA Expression Library," Proc. Natl. Acad. Sci. USA, 83:1573-1577 (1986).
Smith et al. "Hormone Induced Lactation in the Bovine. II. Response of Nulligravida Heifers to Modified Estrogen-Progesterone Treatment," Journal of Dairy Science, 57:296-303 (1973).
Sreekrishna et al., "Construction of Strains of Saccharomyces Cerevisiae that Grow on Lactose," Proc. Natl. Acad. Sci. USA, 82:7909-7913 (1985).
Violotte et al., "Complete Nucleotide Sequence of Bovine .alpha.-lactalbumin Gene: Comparison with its Rat Counterpart," Biochimie, 69:609-620 (1987).
Zhu, et al. "Systemic Gene Expression After Intravenous DNA Delivery into Adult Mice," Science, 261:209-211 (1993).
Felgner et al (1987) Proced. Natl. Acad. Sci. 84, 7413-7417.

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