Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Reexamination Certificate
2011-06-28
2011-06-28
Noakes, Suzanne M (Department: 1656)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
C435S320100, C435S252300, C435S252330, C530S412000, C530S416000
Reexamination Certificate
active
07968311
ABSTRACT:
Expression systems are disclosed for the direct expression of peptide products into the culture media where genetically engineered host cells are grown. High yield was achieved with novel vectors, a special selection of hosts, and/or fermentation processes which include careful control of cell growth rate, and use of an inducer during growth phase. Special vectors are provided which include control regions having multiple promoters linked operably with coding regions encoding a signal peptide upstream from a coding region encoding the peptide of interest. Multiple transcription cassettes are also used to increase yield. The production of amidated peptides using the expression systems is also disclosed.
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Consalvo Angelo P
Meenan Christopher P
Mehta Nozer M
Ray Martha V
Noakes Suzanne M
Ostrolenk Faber LLP
Tsay Marsha M
Unigene Laboratories Inc.
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