Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1998-04-15
2000-08-15
Carlson, Karen Cochrane
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4353201, 4352573, 435 694, 43525233, 530300, 530399, 530412, 530416, C12P 2106, C12N 1500, C12N 112, A61K 3824, A23J 100
Patent
active
061034959
ABSTRACT:
Expression systems are disclosed for the direct expression of peptide products into the culture media where genetically engineered host cells are grown. High yield was achieved with novel vectors, a special selection of hosts, and/or fermentation processes which include careful control of cell growth rate, and use of an inducer during growth phase. Special vectors are provided which include control regions having multiple promoters linked operably with coding regions encoding a signal peptide upstream from a coding region encoding the peptide of interest. Multiple transcription cassettes are also used to increase yield. The production of amidated peptides using the expression systems is also disclosed.
REFERENCES:
patent: 4595658 (1986-06-01), Zinder et al.
patent: 4620948 (1986-11-01), Builder et al.
patent: 4708934 (1987-11-01), Gilligan et al.
patent: 4757013 (1988-07-01), Inouye et al.
patent: 5089406 (1992-02-01), Williams et al.
patent: 5223407 (1993-06-01), Wong et al.
patent: 5240831 (1993-08-01), Barnes
patent: 5332664 (1994-07-01), Craig et al.
patent: 5547862 (1996-08-01), Meador et al.
patent: 5589364 (1996-12-01), Williams et al.
patent: 5747281 (1998-05-01), Shuler et al.
Koke et al. High-Level expression in Escherichia coli and Rapid Purification of Phosphatidylinositol-Specific Phospholipase C from Bacillus cereus and Bacillus thuringiensis. Protein Expression & Purification, 2: 51-58, 1991.
Ray, et al. Bio/Technology, 11:64-70 (1993).
Watson, Nucleic Acids Research, 12(13):5145-5164.
Hsiung, et al., Biotechnology, 4:991-995 (1986).
Vaara, Microbiological Reviews, 56:395-411 (1992).
Kaderbhai, et al., Biotech. Appl. Biochem., 25:53-61 (1997).
Nagahari, et al., EMBO J., 4(13A):3589-3592 (1987).
Miller, et al., ABB, 298:380-388 (1992).
Mizuno, et al., BBRC, 148(2):546-552 (1987).
Lin Ying, et al., Chin. Med. Sci. J., 11(1):204-208 (1996).
Costello, et al., Journal of Bacteriology, 178(6):1623-1630 (1996).
Fujiyama, et al., FEMS Microbiology Letters, 126:19-24 (1995).
Hasan, et al., Gene, 56:145-151 (1987).
Ito, et al., Gene, 118:87-91 (1992).
Murakami, et al., Applied Microbiology and Biotechnology, 619-623 (1989).
Nakamura, et al., EMBOJ. 1(6), 771-775 (1982).
Perez, et al., Bio/Technology, 12:178-180 (1994).
Perez, et al., Journal of Biotechnology, 49:245-247 (1996).
Yu, et al., Biotechnology Letters, 13(5):311-316 (1991).
Dykxhoorn, et al., Gene, 177:133-136 (1996).
Consalvo Angelo P.
Meenan Christopher P.
Mehta Nozer M.
Ray Martha V. L.
Carlson Karen Cochrane
Schnizer Holly
Unigene Laboratories Inc.
LandOfFree
Direct expression of peptides into culture media does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Direct expression of peptides into culture media, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Direct expression of peptides into culture media will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2005226