Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Method of regulating cell metabolism or physiology
Reexamination Certificate
2005-03-21
2008-11-18
Ton, Thaian N (Department: 1632)
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Method of regulating cell metabolism or physiology
C435S383000, C435S384000, C435S363000, C435S366000
Reexamination Certificate
active
07452718
ABSTRACT:
This invention provides a new procedure for generating cardiomyocyte lineage cells from embryonic stem cells for use in regenerative medicine. Differentiating by way of embryoid body formation or in serum is no longer required. Instead, the stem cells are plated onto a solid substrate, and differentiated in the presence of select factors and morphogens. After enrichment for cells with the appropriate phenotype, the cells are allowed to cluster into cardiac bodies™, which are remarkably homogeneous and suitable for the treatment of heart disease.
REFERENCES:
patent: 5733727 (1998-03-01), Field
patent: 5843780 (1998-12-01), Thomson
patent: 5928943 (1999-07-01), Franz et al.
patent: 6015671 (2000-01-01), Field
patent: 6099832 (2000-08-01), Mickle et al.
patent: 6110459 (2000-08-01), Mickle et al.
patent: 6245566 (2001-06-01), Gearhart et al.
patent: 6261836 (2001-07-01), Cech et al.
patent: 6387369 (2002-05-01), Pittenger et al.
patent: 6399300 (2002-06-01), Field
patent: 6534052 (2003-03-01), Xiao et al.
patent: 2002/0061837 (2002-05-01), Lough, Jr. et al.
patent: 2002/0142457 (2002-10-01), Umezawa et al.
patent: 2005/0037489 (2005-02-01), Gepstein et al.
patent: 2005/0054092 (2005-03-01), Xu et al.
patent: 2005/0227353 (2005-10-01), Mummery
patent: 729377 (2001-02-01), None
patent: WO 92/13066 (1992-08-01), None
patent: WO 95/14079 (1995-05-01), None
patent: WO 99/49015 (1999-09-01), None
patent: WO 00/06701 (2000-02-01), None
patent: WO 00/70021 (2000-11-01), None
patent: WO 00/78119 (2000-12-01), None
patent: WO 01/22978 (2001-04-01), None
patent: WO 01/048151 (2001-07-01), None
patent: WO 01/51616 (2001-07-01), None
patent: WO 01/53465 (2001-07-01), None
patent: WO 01/68814 (2001-09-01), None
patent: WO 02/09650 (2002-02-01), None
patent: WO 02/13760 (2002-02-01), None
patent: WO 02/19893 (2002-03-01), None
patent: WO 02/30206 (2002-04-01), None
patent: WO 03/006950 (2003-01-01), None
patent: WO 2004/081205 (2004-09-01), None
Verfaillie et al. Hematology. Am Soc Hematol Educ Program). 2002;:369-91.
Caspi and Gepstein. Ann. NY Accad. Sci., 1015: 285-298, 2004.
Alsan et al., Regulation of avian cardiogenesis by Fgf8 signaling, Development, 129:1935 (2002).
Andree et al., BMP-2 induces expression of cardiac lineage markers and interferes with somite formation in chicken embryos, Mech. of Deve., 70:119 (1998).
Antin et al., Regulation of avian precardiac mesoderm development by insulin and insulin-like growth factors, J. Cell. Physiol. 168:42 (1996).
Arai et al., Murine cardiac progenitor cells require visceral embryonic endoderm and primitive streak for terminal differentiation, Dev. Dynamics 210:344 (1997).
Barron et al., Repuirement for BMP and FGF signaling during cardiogenic induction in non-precrdiac mesoderm is specific, transient, and cooperative, Dev. Dynamics 218:383 (2000).
Bauwens et al., Development of perfusion fed bioreactor for embryonic stem cell-derived cardiomyocyte generation: oxygen-mediated enhancement of cardiomyocyte output, biotechnology and bioengineering 90 (4):452 (2005).
Behfar et al., Stem cell differentiation requires a paracrine pathway in the heart, FASEB J, 16:1558 (2002).
Claycomb et al., HL-1 cells: A cardiac muscle cell line that contracts and retains phenotypic characteristics of the adult cardiomyocyte, Proc. Natl. Acad. Sci. USA 95:2979 (1998).
Dang et al. Controlled, scalable embryonic stem cell differentiation culture, Stem Cells, 22:275 (2004).
Doevendans et al., Differentiation of cardiomyocytes in floating embryoid bodies is comparable to fetal cardiomyocytes, J. Mol. Cell Cardiol, 32:839 (2000).
Dubus et al., Contractile protein gene expression in serum-free cultured adult rat cardiac myocytes, Pflugers Arch, 423:455 (1993).
Fukuda, Development of regenerative cardiomyocytes from mesenchymal stem cells for cardiovascular tissue engineering, Artificial Organs 25:187 (2001).
Gepstein, Derivation and Potential Applications of Human Embronic Stem Cells, Circulation Research, 91 (10):866 (2002).
Grepin et al., Enhanced cardiogenesis in embryonic stem cells overexpressing the GATA-4 transcription factor, Development 124:2387 (1997).
Gryschenko et al., Outwards currents in embryonic stem cell-derived cardiomyocytes, Pflugers Arch. 439:798 (2000).
Heng et al., Strategies for directing the differentiaion of stem cells into the cardiomyogenic lineage in vitro, Cardiovascular Research, 62:34 (2004).
Itskovitz-Eldor et al., Differentiation of Human Embronic Stem Cells Into Embryoid Bodies Comprising the Three Emryonic Germ Layers, Mol. Med. 6:88 (2000).
Johansson et al., Evidence for involvement of activin A and bone morphogenetic protein 4 mammalian mesoderm and hematopoietic development, Mollecular and Cellular Biology, 15(1):141 (1995).
Kawai et al., Efficient cardiomyogenic differentiation of embryonic stem cell by fibroblast growth factor 2 and bone morphogenetic protein 2, Circ J 68:691 (2004).
Kehat e al., Electromechanical integration of cardiomyocytes derived from human embryonic stem cells, Nature Biotechnology, 22(10):1282 (2004).
Kehat et al., Abstract , Human embryonic stem cells can diffrentiate into cyocytes with structural and functional properties of cardiomyocytes, J. Clin. Invest., 108:407 (2001).
Kehat et al., Long term high-resolution, Electrophysiological assessment of human embryonic stem cell derived cardiomyocytes: A novel in vitro model for the human heart, Circulation, 102(18 Suppl. II):II-4 (2000).
Kessler et al., Myoblast cell grafting into heart muscle: Cellular biology and potential applications, Annu. Rev. Physiol. 61:219 (1999).
Klug et al. Genetically selected cardiomyocytes fron differentiating embryonic stem cells form stable intracardiac grafts, J. Clin. Invest. 98:216 (1996).
Koide et al., Atrial natriuretic peptide accelerates proliferation of chick embryonic cardiomyocytes in vitro, Differentiation 61:1 (1996).
Kolossov et al., Functional characteristics of ES cell-derived cardiac precursor cells identified by tissue-specific expression of the green fluorescent protein, J. Cell Biol. 143:2045 (1998).
Ladd et al., Regulation of avian cardiac myogenesis by activin/TGFB and bone morphogenetic proteins, Dev. Biology 204:407 (1998).
Lev et al., Differentiation pathways in human embryonic stem cell-derived cardiomyocytes, Ann. N.Y. Acad. Sci. 1047:50 (2005).
Li et al., Isolation of cardiomyocytes from human myocardium for primary cell culturing, J. Tiss. Cult. Meth. 15:147 (1993).
Liechty et al., Human mesenchymal stem cells engraft and demonstrate site-specific differentiation after in utero transplantation In sheep, Nature Med. 6:1282 (2000).
Lough et al., Combined BMP-2 and FGF-4, but neither factor alone, induces cardiogenesis in non-precardiac embryonic mesoderm, Dev. Biology 178:198 (1996).
Makino et al., Cardiomyocytes can be generated from marrow stromal cells in vitro, J. Clin. Invest. 103:697 (1999).
Maltsev et al., Embryonic stem cells differentiate in vitro into cardiomyocytes representing sinusnodal, atrial and ventricular cell types, Mechanisms Dev. 44:41 (1993).
Matsushita et al., Formation of cell junctions between grafted and host cardiomyocytes at the border zone of rat myocardial infraction, Circulation 100[suppl. II]: II-262 (1999).
Marvin et al., Inhibition of Wnt activity induces heart formation from posterior mesoderm, Genes Dev. 15:316 (2001).
McBurney et al., Control of muscle and neuronal differentiation in a cultured embryonal carcinoma cell line, Nature 299:165 (1982).
McDowell et al., Activin as a morphogen inXenopus mesoderminduction, Seminars in Cell & Development Biology, 10:311 (1999).
Menard et al., Transplantation of cardiac-committed mouse embryonic stem cells to infarcted sheep myocardium: preclinical study, Lancet 366:1005 (2005).
Messina et al., Isolation and expansion of adult ardiac stem cells from human and murine heart , 95:911 (2004).
Min et al., Transplantation of embryonic stem cells improves cardiac function in postinfacted rats, J. Appl. Physiol. 92:288 (2002).
Monzen et al., Bone morphogenetic proteins induce cardiomyocyte differentiation through the
Collins Lila R.
Gold Joseph D.
Hassanipour Mohammad
Xu Chunhui
Geron Corporation
Mittler E. Stewart
Ton Thaian N
LandOfFree
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