Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2006-09-26
2006-09-26
Leith, Patricia (Department: 1655)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C435S004000
Reexamination Certificate
active
07112411
ABSTRACT:
An improved method for detecting a target biomolecule directly in a polyacrylamide gel in which it has been separated from other substances. The improvement resides in, prior to binding the target to a probe and while the target biomolecule remains in the gel, (1) immersing the gel in a water miscible, aqueous extracting medium to shrink the gel by at least about ten percent and then (2) washing the gel with water to restore the gel to substantially its original size.
REFERENCES:
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patent: 2001/0044108 (2001-11-01), Shih et al.
Mohamed et al. Polyacrylamide Gel Miniaturization Improves Protein Visualization and Autoradiographic Detection; Analytical Biochemistry, vol. 177, (1989) pp. 287-290.
Bronstein et al. Clinical Applications of Luminescent Assays Foe Enzymes and Enzyme Labels; Journal of Clinical Laboratory Analysis, vol. 3, No. 5 (1989) pp. 316-322.
Desai et al., “Direct Immunodetection of Antigens within the Precast Polyacrylamide Gel,”Analytical Biochemistry, 297:94-98 (2001).
Mohamed et al., “Polyacrylamide Gel Miniaturization Improves Protein Visualization and Autoradiographic Detection,”Analytical Biochemistry, 177:287-290 (1989).
Leith Patricia
Leydig Voit & Mayer Ltd
Martin Paul C.
Pierce Biotechnology, Inc.
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