Direct cloning of DNA fragments

Details Direct cloning of DNA fragments Direct cloning of DNA fragments

1997-06-18

1999-01-05

Degen, Nancy

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 914, 4351721, 4351723, 4353201, C12P 1934, C12N 1564, C12N 1566, C12N 1570

Patent

active

058561447

ABSTRACT:
A vector for the direct cloning of the products of PCR protocol incorporates single nucleotide overhangs at one or both ends of a linearized DNA segment. The single nucleotide overhangs are uracil or inosine residues, as desired, to facilitate cloning of the desired PCR products.

REFERENCES:
patent: 5487993 (1996-01-01), Herrnstadt et al.
Brownstein, Michael J., et al., "Modulation of Non-templated Nucleotide Addition by Taq DNA Polymerase: Primer Modifications that Facilitate Genotyping," BioTechniques, 20(6):1004-1010 (Jun. 1996).
Magnuson, V.L., et al., "Substrate Nucleotide-Determined Non-Templated Addition of Adenine by Taq DNA Polymerase: Implications for PCR-Based Genotyping and Cloning," BioTechniques, 21(4):700-709 (Oct. 1996).
Novy, Robert E., et al., "Perfectly Blunt Cloning: A superior method for cloning PCR products or any DNA," InNovations, 6:7-11 (Dec. 1996).
Testori et al. Direct Cloning of Unmodified PCR Products by Exploiting an Engineered Restriction Site. Gene. 143: 151-152, 1994.
Khan et al. A Simple Ligation Step Improves the Efficiency of T-Overhang Vectors. Trends In Genetics, 10(7): 225-226, Jul. 1994.

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