Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Reexamination Certificate
2007-11-13
2007-11-13
Shibuya, Mark L. (Department: 1639)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
C435S007100, C514S396000
Reexamination Certificate
active
10211455
ABSTRACT:
A method for identifying compounds binding to HCV polymerase comprising the steps of: contacting said HCV polymerase or an analog thereof with a probe formula I:wherein A is O, S, N, NR1, or CR1, wherein R1is defined herein;----- represents either a single or a double bond;R2is selected from: H, halogen, R21, OR21, SR21, COOR21, SO2N(R22)2, N(R22)2, CON(R22)2, NR22C(O)R22or NR22C(O)NR22wherein R21and each R22is defined herein;B is NR3or CR3, wherein R3is defined herein;with the proviso that, when A is not N, then one of A or B is either CR1or CR3,K is N or CR4, wherein R4is defined herein;L is N or CR5, wherein R5has the same definition as R4defined above;M is N or CR7, wherein R7has the same definition as R4defined above;R5is C(Y1)Z wherein Y1is O or S; andZ is N(R6a)R6or OR6, wherein R6ais H or alkyl or NR61R62wherein R61and R62are defined herein; and R6is H, alkyl, cycloalkyl, alkenyl, Het, alkyl-aryl, alkyl-Het; or R6iswherein R7and R8and Q are as defined herein;Y2is O or S;R9is H, (C1-6alkyl), (C3-7)cycloalkyl or (C1-6)alkyl-(C3-7)cycloalkyl, aryl, Het, (C1-6)alkyl-aryl or (C1-6)alkyl-Het, all of which optionally substituted with R90; or R9is covalently bonded to either of R7or R8to form a 5- or 6-membered heterocycle; or a salt thereof; where the probe comprises a detectable label attached to any suitable position, whereby said probe binds to an HCV polymerase or an analog thereof and is capable of being displaced by an inhibitor thereof.
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Beaulieu Pierre Louis
Kukolj George
McKercher Ginette
Boehringer Ingelheim (Canada) Ltd.
Devlin Mary-Ellen M.
Dow David
Morris Michael
Shibuya Mark L.
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