Direct assay of cholesterol in skin samples removed by tape...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving cholesterol

Reexamination Certificate

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Reexamination Certificate

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10835397

ABSTRACT:
Skin cholesterol is measured by applying an adhesive tape onto a selected area of the skin to adhere the tape to the selected skin area and stripping the tape off the selected skin area to obtain a sample representative of the outer stratum corneum layer of the skin, the sample adhering to the tape so as to have exposed skin constituents. The sample is assayed using a detector reagent that specifically binds to cholesterol and in addition has an indicator component that allows quantitation of the amount of cholesterol present in the exposed skin constituents.

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