Stock material or miscellaneous articles – Coated or structually defined flake – particle – cell – strand,... – Particulate matter
Patent
1995-06-07
1998-03-03
Nakarani, D. S.
Stock material or miscellaneous articles
Coated or structually defined flake, particle, cell, strand,...
Particulate matter
428403, 428407, 548405, B32B 2718
Patent
active
057232180
ABSTRACT:
The invention is a novel fluorescently labeled microparticle, where the microparticle internally incorporates at least one dipyrrometheneboron difluoride dye. Appropriate selection of substituents results in dipyrrometheneboron difluoride derivatives that, when incorporated into polymer microparticles, give the desired excitation and emission wavelengths. The spectral characteristics of the labeling dyes in liquid are not greatly changed when the dye is incorporated into the particles, and the spectral excitation and emission wavelengths are compatible with commonly used filter sets. Other embodiments of the fluorescent microparticles include additional dyes and/or bioreactive substances.
REFERENCES:
patent: 2994697 (1961-08-01), Eckert et al.
patent: 3096333 (1963-07-01), Wilson et al.
patent: 3857931 (1974-12-01), Hager
patent: 4046723 (1977-09-01), Dorman
patent: 4326008 (1982-04-01), Rembaum
patent: 4421896 (1983-12-01), Dorman
patent: 4774339 (1988-09-01), Haugland et al.
patent: 4808524 (1989-02-01), Snyder et al.
patent: 4916711 (1990-04-01), Boyer et al.
patent: 4945171 (1990-07-01), Haugland et al.
patent: 5187288 (1993-02-01), Kang et al.
patent: 5227487 (1993-07-01), Haugland et al.
patent: 5248782 (1993-09-01), Haugland et al.
patent: 5268486 (1993-12-01), Waggoner et al.
patent: 5274113 (1993-12-01), Kang et al.
patent: 5316906 (1994-05-01), Haugland et al.
patent: 5321130 (1994-06-01), Yue et al.
patent: 5326692 (1994-07-01), Brinkley et al.
patent: 5338854 (1994-08-01), Kang et al.
patent: 5405975 (1995-04-01), Kuhn et al.
patent: 5410030 (1995-04-01), Yue et al.
patent: 5433896 (1995-07-01), Kang et al.
Jett, Keller, Martin, Nguyen, & Saunders, Ultrasensitive Molecular-Level Flow Cytometry, Flow Cytometry and Sorting, p. 381 (2nd ed., Wiley-Liss Inc., NY 1990).
Hook & Odeyale, Confocal Scanning Fluorescence Microscopy: A New Method for Phagocytosis Research, J. Leukocyte Biol., 45, 277 (1989).
Carrington, Fogarty, Lifshitz, & Fay, Three-Dimensional Imaging on Confocal and Wide-Field Microscopes, Handbook of Biological Confocal Microscopy, Ch. 14, p. 154 (Rev. ed. J. Pawley, Plenum Press, NY 1990).
Treibs & Kreuzer, Difluorboryl-Komplexe von Di-und Tripyrrylemethenen, Liebigs Ann. Chem., 718, 208 (1968).
Bangs, Uniform Latex Particles, Ch. VII (1984 Seragen, Inc.).
Guilford, Chemical Aspects of Affinity Chromatography, Chem. Soc. Rev., 2, 249 (1973).
Nathan & Cohn, Antitumor Effects of Hydrogen Peroxide in Vivo, J. Exp. Med., 154, 1539-1553 Nov. 1981.
Pethig, Kuhn, Payne, Adler, Chen, & Jaffee, On the Dissociation Constants of BAPTA-type Calcium Buffer, Cell Calcium 10, 491 (1989).
Brinkley John Michael
Haugland Richard P.
Haugland Rosaria P.
Kang Hee Chol
Kuhn Michael
Helfenstein Allegra J.
Molecular Probes, Inc.
Nakarani D. S.
Skaugset Anton E.
LandOfFree
Dipyrrometheneboron difluoride labeled flourescent microparticle does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Dipyrrometheneboron difluoride labeled flourescent microparticle, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Dipyrrometheneboron difluoride labeled flourescent microparticle will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2246043