Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Peptide containing doai
Patent
1995-06-07
1997-10-28
Marschel, Ardin H.
Drug, bio-affecting and body treating compositions
Designated organic active ingredient containing
Peptide containing doai
436501, 436 63, 530300, 530350, 5303871, A61K 3816
Patent
active
056818106
ABSTRACT:
This invention relates to a modified diphtheria toxin (DT) and method of preparing the same in which two carboxy-terminal truncated forms of DT are prepared by specific chemical proteolysis generating two new proteins HA51DT and HA48DT which can be chemically linked to a cell specific binding moiety to produce potent cytotoxins. This invention further relates to carboxy terminal peptides formed in accordance with said proteolysis generating three peptides HA11DT, HA7DT and HA3DT.
REFERENCES:
patent: 4033819 (1977-07-01), Helting
patent: 4379145 (1983-04-01), Masuho et al.
patent: 4620948 (1986-11-01), Builder et al.
patent: 4664911 (1987-05-01), Uhr et al.
patent: 4671958 (1987-06-01), Rodwell et al.
patent: 4675382 (1987-06-01), Murphy
patent: 5208021 (1993-05-01), Johnson et al.
Bishai et al. (Apr. 1987) "Cloning and Expression in Escheria coli of Three Fragments of Diphtheria Toxin Truncated with Fragment B" J. Bacteriol. 169(4):1554-1563.
Colombatti et al. (1986) "Cloned Fragment of Diphtheria Toxin Linked to T Cell-specific Antibody Identifies Regions of B Chain Active in Cell Entry" J. Biol. Chem. 261(7):3030-3035.
Kaczorek et al. (1983) "Nucleotide Sequence and Expression of the Diphtheria tox228 Gene in Escheria coli" Science 221:855-858.
Bornstein et al. (1977) Meth. Enzymol. 47:132-145.
Murphy et al. (1986) "Genetic construction, expression, and melanoma-selective cytotoxicity of a diphtheria toxin-related .alpha.-melanocyte-stimulating hormone fusion protein" Proc. Natl. Acad. Sci. 83:8258-8262.
Knuth et al. (1987) "Purification of Proteins in the Denatured State" in Protein Purification: Micro to Macro (R. Burgess Ed.) Alan R. Liss, Inc., pp. 279-305.
Pharmacia (1984) "Gel Filtration Theory and Practice" Rahms I. Lund, Sweden, pp. 7-11.
Falmagne et al. (1985) "The Complete Amino Acid Sequence of Diphtheria Toxin Fragment B. Correlation with its Lipid-Binding Properties" Biochim. Biophys. Acta 827:45-50.
Howell et al. (1984) "Toxicity of Ricin, Diphtheria Toxin and .alpha.-Amanitin for Acanthamoeba castellanii" J. Parasit. 70(6):918-923.
Villemez et al. (1984) "Preparation of an Immunotoxin for Acanthamoeba castellanii" Biochem. Biophys. Res. Commun. 125(1):25-29.
Sundan et al. (1982) "Preparation and Properties of Chimeric Toxins Prepared from the Constituent Polypeptides of Diphtheria Toxin and Ricin" J. Biol. Chem. 257(16):9733-9739.
Gilliland et al. (1980) "Antibody-Directed Cytotoxic Agents: Use of Monoclonal Antibody to Direct the Action of Toxin A Chains to Colorectal Carcinoma Cells" Proc. Natl. Acad. Sci. USA 77(8):4539-4543.
Boquet (1977) "Transport of Diphtheria Toxin Fragment A Across Mammalian Cell Membranes" Biochem. Biophys. Res. Commun. 75(3):696-702.
Falmagne et al. (1982) "Structure-Activity Relationships of the B Fragment of Diphtheria Toxin: The Lipid-Binding Domains" Toxicon 20(1):243-246.
DeLange et al. (1979) "The Amino Acid Sequence of Fragment A, an Enzymically Active Fragment of Diphtheria Toxin" J. Biol. Chem. 254(13):5838-5842.
DeLange et al. (1979) "The Amino Acid Sequence of Fragment A, an Enzymically Active Fragement of Diphtheria Toxin" J. Biol. Chem. 254(13):5827-5831.
Drazin et al. (1979) "The Amino Acid Sequence of Fragment A, an Enzymically Active Fragment of Diphtheria Toxin" J. Biol. Chem. 254(13):5832-5837.
Chang (1985) "The Effect of Diphtheria Toxin B Chain on Toxicity of the Native Toxin and its A Chain conjugate to Asialoorosomucoid" Fed. Proc.; Toxins I, Monday PM, 1544.
Audibert et al. (1981) "Active Antitoxic Immunization by a Diptheria Toxin Synthetic Oligopeptide" Nature 289:593.
Lambotte et al. (1980) "Primary Structure of Diphtheria Toxin Fragment B: Structural Similarities with Lipid-Binding Domains" J. Cell. Biol. 87:837-840.
Chemical Abstracts (1975) 82:145 No. 166120(g) for Monier (1974) "Tryptic cleavage of the citraconylated B. fragment of diphtheria toxin" Arch. Int. Physiol. Biochim. 82(4):803.
Myers et al. (1988) "Specific chemical cleavage of diphtheria toxin with hydroxylamine" J. Biol. Chem. 263(32):17122-17127.
Williams et al. (1987) "Diphtheria toxin receptor binding domain substitution with interleukin-2: genetic construction and properties of a diphtheria toxin-related interleukin-2 fusion protein" Protein Engineering 1(6):493-498.
Myers Dean A.
Villemez Clarence L.
Marschel Ardin H.
University of Wyoming
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