Diphenylhydantoin antibodies

Details Diphenylhydantoin antibodies Diphenylhydantoin antibodies

1978-12-07

1980-07-22

Phillips, Delbert R.

Drug, bio-affecting and body treating compositions

Lymphokine

2601125R, 536 4, A61K 3900, C07C10352, A61K 4502

Patent

active

042139643

ABSTRACT:
Reagents for use in binding assays, particularly immuno assays, to determine diphenylhydantoin and for preparing reagents employed in such assays, including .beta.-galactosyl-umbelliferone-diphenylhydantoin conjugates, diphenylhydantoin immunogens, and N.sup.1, N.sup.3 and .omicron.-phenyl derivatives of diphenylhydantoin for preparing same. N.sup.1 and N.sup.3 -.omega.-aminoalkyl and .omicron.-(.omega.-aminoalkoxy)-phenyl derivatives are prepared and coupled by amide linkage to a .beta.-galactosyl-umbelliferone derivative to form labeled conjugates useful in homogeneous or heterogeneous binding assays. N.sup.1 and N.sup.3 -.omega.-carboxyalkyl and .omicron.-(.omega.-carboxyalkoxy)-phenyl derivatives are prepared and coupled by amide linkage to an immunogenic polyamino acid to form immunogen conjugates against which diphenylhydantoin-specific antibodies can be raised.

REFERENCES:
patent: 3905871 (1975-07-01), Rubenstein et al.
patent: 3995021 (1976-11-01), Gross
patent: 4092479 (1978-05-01), Parson, Jr. et al.

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