Dipeptidase, its isolation from lactic acid bacteria, antibodies

Details Dipeptidase, its isolation from lactic acid bacteria, antibodies Dipeptidase, its isolation from lactic acid bacteria, antibodies

1988-03-31

1989-12-19

Rosenberg, Peter D.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

435212, 435814, 435853, C12N 900

Patent

active

048882844

ABSTRACT:
This invention relates to dipeptidase of lactic acid bacteria in isolated form, characterized by a molecular weight of 55 kD.+-.5 kD; an isoelectric point of 4.4.+-.0.4; a substrate range including the dipeptides leu-leu, leu-met, leu-val, leu-gly, val-leu, phe-leu and ala-ala, but not the dipeptides his-leu, .gamma.-glu-leu, gly-leu, .alpha.-glu-ala and peptides of 3 or more amino acid residues; and a hydrolyzing activity with a temperature optimum at 50.degree. C..+-.10.degree. C., a pH optimum at 8.+-.1, and sensitivity to the metal chelating EDTA and to the reducing agents dithiotreitol and mercaptoethanol. The invention also relates to a process for isolating the dipeptidase from lactic acid bacteria; to the use of the dipeptidsae for preparing polyclonal or monoclonal antibodies having an affinity to, and/or specificity for, the dipeptidase; to polyclonal and monoclonal antibodies having an affinity to, and/or specificity for, the dipeptidase; to the use of polyclonal or monoclonal antibodies for detecting the dipeptidase in, or removing or isolating it from, a mixture containing the dipeptidase; to the use of the dipeptidase in the production of food products, and to food products produced using the dipeptidase.

REFERENCES:
patent: 3947324 (1976-03-01), Lakshminarayanan
patent: 4560661 (1985-12-01), Katsumata

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