Dimeric glycerol dehydratases

Details Dimeric glycerol dehydratases Dimeric glycerol dehydratases

2010-01-04

2010-12-28

Fronda, Christian L (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Lyase

C435S155000, C435S183000, C435S252300, C435S320100, C536S023200

Reexamination Certificate

active

07858355

ABSTRACT:
The invention concerns a method for preparing 1,3-propanediol from a carbon-containing substance, said method comprising a step which consists in culturing a recombinant micro-organism not producing coenzyme B12 in the absence of coenzyme B12 or one of its precursors. The invention also concerns a nucleic acid coding for a glycerol dehydratase whereof the catalytic activity is independent of the presence of coenzyme B12 or one of its precursors and a nucleic acid coding for a 1,3-propanol dehydrogenase intervening in the synthesis of 1,3-propanediol. The invention further concerns recombinant vectors and host cells comprising said nucleic acids and the polypeptides coded by the latter.

REFERENCES:
patent: 5633362 (1997-05-01), Nagarajan et al.
patent: 5686276 (1997-11-01), Laffend et al.
patent: 5821092 (1998-10-01), Nagarajan et al.
patent: 7267972 (2007-09-01), Sarcabal et al.
patent: 2009/0029431 (2009-01-01), Sarcabal et al.
patent: WO 98/21339 (1998-05-01), None
Chica et al. Curr Opin Biotechnol. Aug. 2005;16(4):378-84.
Sen et al. Appl Biochem Biotechnol. Dec. 2007;143(3):212-23.
Chica et al., “Semi-rational approach to engineering enzyme activity: combining the benefits of directed evolution and rational design,”Curr. Opin. Biotechnol., 16 (4): 378-384, 2005.
Luers et al., “Glycerol conversion to 1,3-propanediol byClostridium pasteurianum: cloning and expression of the gene encoding 1,3-propanediol dehydrogenase,”FEMS Microbiology Letters, 154:337-345, 1997.
Macis et al., “Properties and sequences of the coenzyme B12-dependent glycerol dehydratase ofClostridium pasteurianum,” FEMS Microbiology Letters, 164:21-28, 1998.
Office Action, issued in U.S. Appl. No. 10/043,639, mailed Nov. 1, 2006.
Office Action, issued in U.S. Appl. No. 10/043,639, mailed Apr. 20, 2006.
Office Action, issued in U.S. Appl. No. 10/043,639, mailed Jul. 29, 2005.
Office Action, issued in U.S. Appl. No. 10/043,639, mailed Mar. 31, 2005.
Office Action, issued in U.S. Appl. No. 10/043,639, mailed Jun. 23, 2004.
Office Action, issued in U.S. Appl. No. 11/839,455, mailed Feb. 20, 2009.
Reimann et al., “1,3-propanediol formation with product-tolerant mutants ofCostridium butyricumDSM 5431 in continuous culture: productivity, carbon and electron flow,”J. Applied Microbiology, 84:1125-1130, 1998.
Sen et al., “Developments in directed evolution for improving enzyme functions,”Appl. Biochem. Biotechnol., 143 (3): 212-223, 2007.
Skraly et al., “Construction and characterization of a 1,3-propanediol operon,”Applied and Environmental Microbiology, 64(1):98-105, 1998.
Weidner and Sawers, “Molecular characterization of the genes encoding pyruvate formate-lyase and its activating enzyme ofClostridium pasteurianum,” J. of Bacteriology, 178(8):2440-2444, 1996.
Raynaud et al., “Molecular characterization of the 1,3-propanediol (1,3-PD) operon ofClostridium butyricum,” Proc. Natl. Acad. Sci. USA, 100(9):5010-5015, 2003.
Seyfried et al., “Cloning, sequencing, and overexpression of the genes encoding coenzyme B12-dependent glycerol dehydratase ofCitrobacter freundii,” Journal of Bacteriology, 178(19):5793-5796, 1996.

Affiliated with

Also associated with

Rating

Dimeric glycerol dehydratases does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Dimeric glycerol dehydratases, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Dimeric glycerol dehydratases will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-4221198