Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Nitrogen containing other than solely as a nitrogen in an...
Patent
1997-03-06
2000-03-07
O'Sullivan, Peter
Drug, bio-affecting and body treating compositions
Designated organic active ingredient containing
Nitrogen containing other than solely as a nitrogen in an...
514 44, 514478, 514479, 514563, 560158, 564153, 564154, A61K 4800, A61K 31195, A61K 3116
Patent
active
060341351
ABSTRACT:
The present invention provides novel dimeric cationic lipids. The present invention further provides compositions of these lipids with anionic or polyanionic macromolecules, methods for interfering with protein expression in a cell utilizing these compositions and a kit for preparing the same.
REFERENCES:
patent: 4897355 (1990-01-01), Eppstein et al.
patent: 5171678 (1992-12-01), Behr et al.
Clivio, et al., Synthesis and Purification of Oligonucleotides Containing Sulfur Substituted Nucleobases: 4-Thiouracil, 4-Thiothymine and 6-Mercaptopurine; Tetrahedron Letters, 33(1):66-68 (1992).
Clivio, et al., Synthesis of Dinucleoside Phosphates Containing Sulfur Substituted Nucleobases: 4-Thiouracil, 4-Thiothymine and 6-Mercaptopurine; Tetrahedron Letters, 33(1)69-72 (1992).
Connolly, et al., Synthesis and properties of oligonucleotides containing 4-thiothymidine, 5-methyl-2-pyrimidinone-1.beta.-D(2'deoxyriboside) and 2-thiothymidine, Nucleic Acids Research, 17(13):4957-4974 (1989).
Felgner, et al., Enhanced Gene Delivery and Mechanism Studies with a Novel Series of Cationic Lipid Formulations, J. Biol. Chem. 269(4):2550-2561 (1994).
Felgner, et al., Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure; Proc. Natl. Acad. Sci. USA, 84:7413-7417 (1987).
Fukunaga, et al., Liposome Entrapment Enhances the Hypocalcemic Action of Parenterally Administered Calcitonin; Endocrinology, 115(2):757-761 (1984).
Gao, et al., A Novel Cationic Lipoxome Reagent for Efficient Transfection of Mammalian Cells; Biochem. and Biophys. Res. Comm., 179(1)280-285 (1991).
Gershon, et al., Mode of Formation and Structural Features of DNA-Cationic Liposome Complexes Used for Transfection; Biochemistry 32:7143-7151 (1993).
Kim, et al., Preparation of Multivesicular Liposomes; Biochimica et Biophysica Acta, 728:339-348 (1983).
Mayer, et al., vesicles of variable sizes produced by a rapid extrusion procedure; Biochimica et Biophysica Acta, 858:161-168 (1986).
Mayhew, et al., Characterization of liposomes prepared using a microemulsifier; Biochimica et Biophysic Acta, 775:169-174 (1984).
Nabel, et al., Gene Transfer In Vivo with DNA-Liposome Complexes: Lack of Autoimmunity and Gonadal Localization; Human Gene Therapy, 3:649-656 (1992).
Nabel, et al., Direct gene transfer with DNA-liposome complexes in melanoma: Expression, biologic activity, and lack of toxicity in humans; Proc. Natl. Acad. Sci. USA, 90:11307-11311 (1993).
Nikiforov, et al., 5'-O-(4,4"Dimethoxytrityl)-4-Thiocyanatothymidine: A Useful Intermediate for the Preparation of Various 4-Substituted Thymidine Analogues; Tetrahedron Letters, 32(22):2505-2508 (1991).
Nikiforov, et al., Straightforward Preparation and Use in Oligodeoxynucleotide Synthesis of 5'-O-(4,4"Dimethoxytrityl)-4-[S-(2-Cyanoethyl)]-Thiothymidine; Tetrahedron Letters, 33(17):2379-2382 (1992).
Olson, et al., Preparation of Liposomes of Defined Size Distribution by Extrusion Through Polycarbonate Membranes; Biochimica et Biophysica Acta, 557:9-23 (1979).
Strauss, et al., Molecular complemantation of a collagen mutation in mammalian cells using yeast artificial chromosomes; EMBO Journal 11(2):417-422 (1992).
Szoka, et al., Procedure for preparation of liposomes with large internal aqueous space and high capture by reverse-phase evaporation; Proc. Natl. Acad. Sci. USA, 75(9):4194-4198 (1978).
Wagner, et al., Antisense Gene Inhibition by Oligonucleotides Containing C-5 Propyne Pyrimidines; Science 260:1510-1513 (1993).
Xu, et al., Simple Synthesis of 4-Thiothymidine, 4-Thiouridine and 6-Thio-2'-deoxyguanosine; Tetrahedron Letters, 32(24):2817-2820(1991).
Zhou, et al., Lipophilic polylysines mediate efficient DNA transfection in mammalian cells; Biochimica et Biophysica Acta, 1065:8-14 (1991).
Brown Bob Dale
Daily William J.
Dwyer Brian Patrick
Schwartz David Aaron
Srinivasan Kumar
Frenchick Grady J.
O'Sullivan Peter
Promega Biosciences, Inc.
LandOfFree
Dimeric cationic lipids does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Dimeric cationic lipids, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Dimeric cationic lipids will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-363766