Dimer and multimer forms of single chain polypeptides

Drug – bio-affecting and body treating compositions – Immunoglobulin – antiserum – antibody – or antibody fragment,...

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

4241331, 4241381, 4241411, 4241521, 4241551, 4241581, 4241721, 4241741, 4241781, 4241811, 435 71, 435 72, 435 723, A61K 39395, G01N 3353, G01N 33574

Patent

active

060715154

ABSTRACT:
The present invention discloses novel proteins which are dimers and multimers of single chain polypeptides. The single chain polypeptides having two domains derived from the immunoglobulin superfamily which are joined by a peptide linker. The dimers and multimers being formed by non-covalent linking of the single chain polypeptides.

REFERENCES:
patent: 4474893 (1984-10-01), Reading et al.
patent: 4642334 (1987-02-01), Moore et al.
patent: 4714681 (1987-12-01), Reading
patent: 4946778 (1990-08-01), Ladner et al.
patent: 5001225 (1991-03-01), Taylor
patent: 5130297 (1992-07-01), Sharma et al.
patent: 5132405 (1992-07-01), Huston et al.
Filpula et al (Antibody Engineering, Eds McCafferty et al., IRL Press, Oxford, UK, pp. 253-268), 1996.
Hunkapiller et al., "Implications of the Diversity of the Immunoglobulin Gene Superfamily", Cold Spring Harbor Symposia on Quantitative Biology, LIV, 1989, pp. 15-29, Cold Spring Harbor Laboratory Press.
Wraith et al., "T-cell Recognition as the Target for Immune Intervention in Autoimmune Disease", Cell, vol. 57, pp. 709-715, Jun. 2, 1989.
Adorini, et al., "New Perspectives on Immunointervention in Autoimmune Diseases", Immunology Today, vol. 11, No. 11, pp. 383-386 (1990).
Bird et al., "Single-Chain Antigen-Binding Proteins", Science, vol. 242, pp. 423-426, Oct. 21, 1988.
Skerra et. al., "Assembly of a Functional Immunoglobulin, F.sub.v Fragment in Escherichia coli", Science, vol. 240, pp. 1038-1041, May 20, 1988.
Takkinen et al., "An Active Single-Chain Antibody Containing a Cellulase Linker Domain is Secreted by Escherichia coli", Protein Engineering, vol. 4, No. 7, pp. 837-841 (1991).
Traunecker et al., "Bispecific Single Chain Molecules (Janusins) Target Cytotoxic Lymphocytes on HIV Infected Cells", The EMBO Journal, vol. 10, No. 12, pp. 3655-3659 (1991).
Huston et al., "Protein Engineering of Antibody Binding Sites: Recovery of Specific Activity in an Anti-digoxin Single-Chain Fv Analogue Produced in Escherichia coli", Proc. Natl. Acad. Sci., Biochemistry, vol. 85, pp. 5879-5883, Aug. 1988.
Larson, "Improved Tumor Targeting With Radiolabeled Recombinant, Single-Chain, Antigen-Binding Protein", Journal of the National Cancer Institute, vol. 82, No. 14, pp. 1173-74, Jul. 18, 1990.
Colcher et al., In Vivo Tumor Targeting of a Recombinant Single-Chain Antigen-Binding Protein, Journal of the National Cancer Institute, vol. 82, No. 14, pp. 1191-1197, Jul. 18, 1990.
Bedzyk et al., "Immunological and Structural Characterization of a High Affinity Anti-fluorescein Single-chain Antibody", The Journal of Biological Chemistry, V. 265, No. 30, pp. 18615-18620, Oct. 25, 1990.
Winter et al., "Man-made Antibodies", Nature, vol. 349, pp. 293-299, Jan. 24, 1991.
Davis, "Single Chain Antibody (SCA) Encoding Genes: One Step Construction and Expression in Eukaryotic Cells", Biotechnology, vol. 9, pp. 165-169, Feb. 1991.
Whitlow et al., "Single-Chain Fv Proteins and Their Fusion Proteins", Methods: A Companion to Methods in Enzymology, vol. 2, No. 2, pp. 97-105, Apr. 1991.
Skerra et al., "The Functional Expression of Antibody F.sub.v Fragments in Escherichia coli: Improved Vectors and a Generally Applicable Purification Technique", Biotechnology, vol. 9, pp. 273-278, Mar. 1991.
Gibbs et al., "Construction and Characterization of a Single-Chain Catalytic Antibody", Proc. Natl. Acad. Sci., U.S.A., vol. 88, pp. 4001-4004, May 1991.
Hodgson, "Making Monoclonals in Microbes", Biotechnology, vol. 9, pp. 421-425, May 1991.
Pluckthun, "Antibody Engineering: Advances From the Use of Escherichia coli Expression Systems", Biotechnology, vol. 9, pp. 545-550, Jun. 1991.
Bird et al., "Single Chain Antibody Variable Regions", Trends in Biotechnology, vol. 9, pp. 132-137 (1991).
Glockshuber et al., "A Comparison of Strategies to Stabilize Immunoglobulin F.sub.v -Fragments", Biochemistry, vol. 29, pp. 1362-1367 (1990).
Denzin et al., "Single-chain Site-specific Mutations of Fluorescein-Amino Acid Contact Residues in High Affinity Monoclonal Antibody 4-4-20", Journal of Biological Chemistry, vol. 266, No. 21, pp. 14095-14103 (1991).
Pantoliano et al., "Conformational Stability, Folding, and Ligand-Binding Affinity of Single-Chain Fv Immunoglobulin Fragments Expressed in Escherichia coli", Biochemistry, vol. 30, pp. 10117-10125 (1991).
Gregoire et al., "Engineered Secreted T-Cell Receptor .alpha..beta. Heterodimers", Proc. Natl. Acad. Sci., U.S.A., vol. 88, pp. 8077-8081, Sep. 1991.
Pack et al., "Miniantibodies: Use of Amphipathic Helices to Produce Functional, Flexibly Linked Dimeric F.sub.v Fragments with High Avidity in Escherichia coli", Biochemistry, vol. 31, No. 6, pp. 1579-1584 (1992).
Soo Hoo et al., "Characterization of a Single-chain T-cell Receptor Expressed in Escherichia coli", Proc. Natl. Acad. Sci., U.S.A., vol. 89, pp. 4759-4763, May 1992.
Novotny et al., "A Soluble, Single-chain T-cell Receptor Fragment Endowed With Antigen-combining Properties", Proc. Natl. Acad. Sci., U.S.A., vol. 88, pp. 8646-8650, Oct. 1991.
Williams et al., "The Immunoglobulin Superfamily-Domains for Cell Surface Recognition", Ann. Rev. Immunol., vol. 6, pp. 381-405 (1988).
Williams et al., "Structural Diversity in Domains of the Immunoglobulin Superfamily", Cold Spring Harbor Symposia on Quantitative Biology, LIV, pp. 637-647, 1989, Cold Spring Harbor Lab. Press.
Bradwel et al (Monoclonal Antibodies for Cancer Detection & Therapy, 1985, Academic Press, London pp. 65-85.
Waldman, Science, 1991, 252:1657-1662.
Sheer et al, Cancer Res, 48: 6811-6818, 1988.
Rodwell et al, Biotechnology, 1985, 3: 889-894.
Hird et al (Genes and Cancer, John Wiley & Sons, 1990, Chichester, pp. 183-189.
Kramer (4.sup.th Annual Symposium, Jan. 23-26, 1992, Diagnostic & Therapeutic Applications in Benign & Malignant Disorders Key Biscayne FL, pp. 52-54, Meeting Abstract.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Dimer and multimer forms of single chain polypeptides does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Dimer and multimer forms of single chain polypeptides, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Dimer and multimer forms of single chain polypeptides will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-2211188

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.