Diluting and dispensing probe for blood sample preparation

Details Diluting and dispensing probe for blood sample preparation Diluting and dispensing probe for blood sample preparation

1977-01-13

1978-08-22

Reeves, Robert B.

Chemistry: physical processes

Physical processes

Crystallization

73423A, 222137, 222145, 222255, G01N 114

Patent

active

041086082

ABSTRACT:
Sample preparation apparatus for forming a thin layer of diluted blood specimen on a substrate by centrifugal force includes a sample probe having coaxial blood and diluent conduits coupled to respective reversibly-drivable peristaltic pumps having forward operation intakes connected to a diluent supply. A one-way clutch limits the diluent pump to forward operation and the pumps are geared to produce a blood-to-diluent displacement ratio of 2:1. A substrate holder is mounted on a carriage for selective insertion into and removal from a sealed centrifuge chamber in which it is spun at a high angular rate by a drive motor. Means are provided to form a curtain of flowing liquid within the chamber surrounding the spinning substrate to collect spun-off blood particles and aerosol. The liquid forming the curtain is drained by an exhaust pump which operates after spinning has ceased so as to create a partial vacuum for draining off residual aerosol prior to unsealing the centrifuge chamber.

REFERENCES:
patent: 1546752 (1925-07-01), Rohr
patent: 3081909 (1963-03-01), Hooker
patent: 3097764 (1963-07-01), Loeser
patent: 3800984 (1974-04-01), Phelan
patent: 3806039 (1974-04-01), Mocarski
patent: 3948605 (1976-04-01), Atwood et al.
patent: 3994687 (1976-11-01), Engelbrecht

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